Literature DB >> 1497336

Detection of Aspergillus and Penicillium extracellular polysaccharides (EPS) by ELISA: using antibodies raised against acid hydrolysed EPS.

H J Kamphuis1, G A De Ruiter, G H Veeneman, J H van Boom, F M Rombouts, S H Notermans.   

Abstract

Species of the fungal genera Aspergillus and Penicillium produce immunologically active extracellular polysaccharides (EPS) in which galactofuranose residues are immunodominant. The antigenic determinant of the EPS of A. fumigatus, A. niger and P. digitatum could be removed by acid hydrolysis. Due to the hydrolysis of the EPS the immunological reaction between IgG anti-native EPS and hydrolysed EPS disappeared. Antibodies raised in rabbits against the acid hydrolysed EPS revealed new antigenic determinants that were exposed as a result of the acid hydrolysis. Immunological inhibitory experiments showed that the antibodies were no longer directed to galactofuranose residues. Enzyme Linked Immunosorbent Assay, carried out with antibodies raised against the acid hydrolysed EPS showed that the antibodies against the acid hydrolysed EPS were more species specific in comparison with the antibodies against the native EPS.

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Year:  1992        PMID： 1497336     DOI： 10.1007/bf00713940

Source DB:  PubMed          Journal:  Antonie Van Leeuwenhoek        ISSN： 0003-6072            Impact factor:   2.271


  15 in total

1.  The effect of variation in molecular weight on the antigenicity of dextran in man.

Authors:  E A KABAT; A E BEZER
Journal:  Arch Biochem Biophys       Date:  1958-12       Impact factor: 4.013

2.  Chemical structures of main extracellular polysaccharides of Alternaria solani and Fusarium solani. Studies on fungal polysaccharides. XVIII.

Authors:  T Miyazaki; Y Naoi
Journal:  Chem Pharm Bull (Tokyo)       Date:  1975-08       Impact factor: 1.645

3.  Immunological relationship of extra-cellular polysaccharide antigens produced by different mould species.

Authors:  S Notermans; P S Soentoro
Journal:  Antonie Van Leeuwenhoek       Date:  1986       Impact factor: 2.271

4.  Peroxidase-labeled antibody. A new method of conjugation.

Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

5.  The 5-O-beta-D-galactofuranosyl-containing exocellular glycopeptide of Penicillium charlesii. Characterization of the phosphogalactomannan.

Authors:  J E Gander; N H Jentoft; L R Drewes; P D Rick
Journal:  J Biol Chem       Date:  1974-04-10       Impact factor: 5.157

6.  Extracellular polysaccharide of Cladosporium herbarum. Studies on fungal polysaccharide. XIII.

Authors:  T Miyazaki; Y Naoi
Journal:  Chem Pharm Bull (Tokyo)       Date:  1974-06       Impact factor: 1.645

Review 7.  The nature of an antigenic determinant.

Authors:  E A Kabat
Journal:  J Immunol       Date:  1966-07       Impact factor: 5.422

8.  Galactofuranosyl groups are immunodominant in Aspergillus fumigatus galactomannan.

Authors:  J E Bennett; A K Bhattacharjee; C P Glaudemans
Journal:  Mol Immunol       Date:  1985-03       Impact factor: 4.407

9.  (1----5)-linked beta-D-galactofuranosides are immunodominant in extracellular polysaccharides of Penicillium and Aspergillus species.

Authors:  S Notermans; G H Veeneman; C W van Zuylen; P Hoogerhout; J H van Boom
Journal:  Mol Immunol       Date:  1988-10       Impact factor: 4.407

10.  Purification and properties of extracellular polysaccharide (EPS) antigens produced by different mould species.

Authors:  S Notermans; G Wieten; H W Engel; F M Rombouts; P Hoogerhout; J H van Boom
Journal:  J Appl Bacteriol       Date:  1987-02
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  5 in total

1.  Increased levels of markers of microbial exposure in homes with indoor storage of organic household waste.

Authors:  I M Wouters; J Douwes; G Doekes; P S Thorne; B Brunekreef; D J Heederik
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

2.  The stability of extracellular beta-glucosidase from Aspergillus niger is significantly enhanced by non-covalently attached polysaccharides.

Authors:  M H Rashid; K S Siddiqui
Journal:  Folia Microbiol (Praha)       Date:  1996       Impact factor: 2.099

3.  Differences among the cell wall galactomannans from Aspergillus wentii and Chaetosartorya chrysella and that of Aspergillus fumigatus.

Authors:  Begoña Gómez-Miranda; Alicia Prieto; Juan Antonio Leal; Oussama Ahrazem; Jesús Jiménez-Barbero; Manuel Bernabé
Journal:  Glycoconj J       Date:  2004       Impact factor: 2.916

4.  New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase.

Authors:  A W Van Bruggen-Van Der Lugt; H J Kamphuis; G A De Ruiter; P Mischnick; J H Van Boom; F M Rombouts
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

Review 5.  Review of methods applicable to the assessment of mold exposure to children.

Authors:  H K Dillon; J D Miller; W G Sorenson; J Douwes; R R Jacobs
Journal:  Environ Health Perspect       Date:  1999-06       Impact factor: 9.031
  5 in total

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