BACKGROUND: We determined the diagnostic value of the trypsin inhibitor, uristatin, that is commonly found in urine and plasma in patients with infections or inflammations of any kind. METHODS: We collected urine specimens from patients with infections of the urinary or upper respiratory tract and from healthy controls. We also collected blood from patients with a likely upper respiratory tract infection and healthy controls. A bacterial count of >10(5) organisms/ml in urine was considered to represent infection rather than contamination. RESULTS: The uristatin dipstick test in urine showed acceptable negative predictive values (NPV of up to 93%) for patients without infection or inflammation. Here, the dipsticks could eliminate some urine cultures. For those with infection or inflammation, the positive predictive values (PPV) of the dipsticks were lower (up to 57%). Including the leukocyte esterase and nitrite values increased the PPV of the dipsticks for those with disease. CONCLUSIONS: The uristatin strip was more accurate than the leukocyte and nitrite dipsticks for predicting upper respiratory infections (URI) and C-reactive protein for those with infection or inflammation. The uristatin dipstick was able to detect both the bikunin and uristatin inhibitors.
BACKGROUND: We determined the diagnostic value of the trypsin inhibitor, uristatin, that is commonly found in urine and plasma in patients with infections or inflammations of any kind. METHODS: We collected urine specimens from patients with infections of the urinary or upper respiratory tract and from healthy controls. We also collected blood from patients with a likely upper respiratory tract infection and healthy controls. A bacterial count of >10(5) organisms/ml in urine was considered to represent infection rather than contamination. RESULTS: The uristatin dipstick test in urine showed acceptable negative predictive values (NPV of up to 93%) for patients without infection or inflammation. Here, the dipsticks could eliminate some urine cultures. For those with infection or inflammation, the positive predictive values (PPV) of the dipsticks were lower (up to 57%). Including the leukocyte esterase and nitrite values increased the PPV of the dipsticks for those with disease. CONCLUSIONS: The uristatin strip was more accurate than the leukocyte and nitrite dipsticks for predicting upper respiratory infections (URI) and C-reactive protein for those with infection or inflammation. The uristatin dipstick was able to detect both the bikunin and uristatin inhibitors.
Authors: Michael J Pugia; Saeed A Jortani; Manju Basu; Ronald Sommer; Hai-Hang Kuo; Solomon Murphy; Doug Williamson; James Vranish; Patrick J Boyle; Danny Budzinski; Roland Valdes; Subhash C Basu Journal: Glycoconj J Date: 2007-01 Impact factor: 2.916
Authors: Saeed A Jortani; Michael J Pugia; Ronald J Elin; Meera Thomas; Edward P Womack; Todd Cast; Roland Valdes Journal: J Clin Lab Anal Date: 2004 Impact factor: 2.352
Authors: Michael J Pugia; Meeta Pradhan; Rong Qi; Doreen L Eastes; Anna Vorsilak; Bradley J Mills; Zane Baird; Aruna Wijeratne; Scott M McAhren; Amber Mosley; Anantha Shekhar; Daniel H Robertson Journal: Arch Autoimmune Dis Date: 2020