Literature DB >> 14963838

A technique to obtain fibroblast cells from skin biopsies of living bats (Chiroptera) for cytogenetic studies.

Ricardo Moratelli1, Cláudio de Moraes Andrade, Jorge Luís Azevedo de Armada.   

Abstract

We developed a procedure to obtain fibroblasts from bat skin. A small fragment of the ear is removed under ether anesthesia. This material is then cut up into small pieces and cultured in standard cell culture media. Very good quality chromosome preparations for cytogenetic studies are obtained in about three weeks. Secondary cultures can be used for other biological studies. This procedure does not require sacrificing the animals.

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Mesh：

Year:  2002        PMID： 14963838

Source DB:  PubMed          Journal:  Genet Mol Res        ISSN： 1676-5680


  6 in total

1.  A phylogenetic analysis using multidirectional chromosome painting of three species (Uroderma magnirostrum, U. bilobatum and Artibeus obscurus) of subfamily Stenodermatinae (Chiroptera-Phyllostomidae).

Authors:  J C Pieczarka; A J B Gomes; C Y Nagamachi; D C C Rocha; J D Rissino; P C M O'Brien; F Yang; M A Ferguson-Smith
Journal:  Chromosome Res       Date:  2013-06-18       Impact factor: 5.239

2.  Phylogenetic reconstruction by cross-species chromosome painting and G-banding in four species of Phyllostomini tribe (Chiroptera, Phyllostomidae) in the Brazilian Amazon: an independent evidence for monophyly.

Authors:  Talita Fernanda Augusto Ribas; Luis Reginaldo Ribeiro Rodrigues; Cleusa Yoshiko Nagamachi; Anderson José Baia Gomes; Jorge das Dores Rissino; Patricia Caroline Mary O'Brien; Fengtang Yang; Malcolm Andrew Ferguson-Smith; Julio Cesar Pieczarka
Journal:  PLoS One       Date:  2015-03-25       Impact factor: 3.240

3.  Karyotype, evolution and phylogenetic reconstruction in Micronycterinae bats with implications for the ancestral karyotype of Phyllostomidae.

Authors:  T C M Benathar; C Y Nagamachi; L R R Rodrigues; P C M O'Brien; M A Ferguson-Smith; F Yang; J C Pieczarka
Journal:  BMC Evol Biol       Date:  2019-05-07       Impact factor: 3.260

4.  Karyotypic variation in Rhinophylla pumilio Peters, 1865 and comparative analysis with representatives of two subfamilies of Phyllostomidae (Chiroptera).

Authors:  Anderson José Baia Gomes; Cleusa Yoshiko Nagamachi; Luís Reginaldo Ribeiro Rodrigues; Solange Gomes Farias; Jorge Dores Rissino; Julio Cesar Pieczarka
Journal:  Comp Cytogenet       Date:  2012-05-23       Impact factor: 1.800

5.  Chromosomal phylogeny of Vampyressine bats (Chiroptera, Phyllostomidae) with description of two new sex chromosome systems.

Authors:  Anderson José Baia Gomes; Cleusa Yoshiko Nagamachi; Luis Reginaldo Ribeiro Rodrigues; Thayse Cristine Melo Benathar; Talita Fernanda Augusto Ribas; Patricia Caroline Mary O'Brien; Fengtang Yang; Malcolm Andrew Ferguson-Smith; Julio Cesar Pieczarka
Journal:  BMC Evol Biol       Date:  2016-06-04       Impact factor: 3.260

6.  Chromosomal evolution and phylogeny in the Nullicauda group (Chiroptera, Phyllostomidae): evidence from multidirectional chromosome painting.

Authors:  Anderson José Baia Gomes; Cleusa Yoshiko Nagamachi; Luis Reginaldo Ribeiro Rodrigues; Malcolm Andrew Ferguson-Smith; Fengtang Yang; Patricia Caroline Mary O'Brien; Julio Cesar Pieczarka
Journal:  BMC Evol Biol       Date:  2018-04-25       Impact factor: 3.260
  6 in total

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