Attenuation of porcine tissues in vivo after high-intensity ultrasound treatment.

The attenuation of ultrasound (US) waves in biologic tissues is an important determinant of energy absorption and wave propagation; thus, important in optimization of high-intensity focused US (HIFU) therapy. We measured attenuation of selected porcine tissues (liver, spleen and abdominal wall) in vivo in the frequency range of 1 to 5 MHz, using the pulse-transmission method, before and after HIFU treatment. In all tissues, an increase in attenuation was observed with increasing frequency. The attenuation coefficient was higher in HIFU-treated tissues than in the untreated tissues. The lowest attenuation was measured in the liver, both in normal and HIFU-treated cases. Mechanisms that may be responsible for the observed attenuation coefficient increase in HIFU-treated tissues include thermally induced change in the tissue macromolecular structure and presence of gas/vapor bubbles due to cavitation and/or boiling.