| Literature DB >> 14962384 |
Oliver Ohlenschläger1, Jens Wöhnert, Enrico Bucci, Simone Seitz, Sabine Häfner, Ramadurai Ramachandran, Roland Zell, Matthias Görlach.
Abstract
Stemloop D (SLD) of the 5' cloverleaf RNA is the cognate ligand of the coxsackievirus B3 (CVB3) 3C proteinase (3Cpro). Both are indispensable components of the viral replication initiation complex. SLD is a structurally autonomous subunit of the 5' cloverleaf. The SLD structure was solved by NMR spectroscopy to an rms deviation of 0.66 A (all heavy atoms). SLD contains a novel triple pyrimidine mismatch motif with a central Watson-Crick type C:U pair. SLD is capped by an apical uCACGg tetraloop adopting a structure highly similar to stable cUNCGg tetraloops. Binding of CVB3 3Cpro induces changes in NMR spectra for nucleotides adjacent to the triple pyrimidine mismatch and of the tetraloop implying them as sites of specific SLD:3Cpro interaction. The binding of 3Cpro to SLD requires the integrity of those structural elements, strongly suggesting that 3Cpro recognizes a structural motif instead of a specific sequence.Entities:
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Year: 2004 PMID： 14962384 DOI： 10.1016/j.str.2004.01.014
Source DB: PubMed Journal: Structure ISSN： 0969-2126 Impact factor: 5.006