AIM: Engineering of Rap-Phr quorum-sensing systems of Bacillus subtilis and subsequent evaluation of the transcription of the aprE gene, encoding a major extracellular alkaline protease. METHODS AND RESULTS: Addition of synthetic Phr pentapeptides to the growth medium, or overproduction of pre-Phr peptides, slightly improved the transcription of the aprE gene in B. subtilis. Disruption of certain rap genes similarly improved the transcription of the aprE gene. The production of extracellular proteolytic enzymes was increased when the rapA mutation was combined with a degU32 (Hy) mutation for hyper-secretion. CONCLUSIONS: Certain Rap-Phr systems of B. subtilis seem to suppress extracellular AprE production. Although this may be an important feature under natural conditions, repression of AprE production by these systems is not desirable under fermentation conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: Although the levels of aprE transcriptional increase in this study are moderate, engineering of Rap-Phr systems may be used to improve the yield of Bacillus strains that are used for the production of the extracellular protease AprE, or Bacillus strains that use of the aprE promoter for the production of a heterologous protein.
AIM: Engineering of Rap-Phr quorum-sensing systems of Bacillus subtilis and subsequent evaluation of the transcription of the aprE gene, encoding a major extracellular alkaline protease. METHODS AND RESULTS: Addition of synthetic Phr pentapeptides to the growth medium, or overproduction of pre-Phr peptides, slightly improved the transcription of the aprE gene in B. subtilis. Disruption of certain rap genes similarly improved the transcription of the aprE gene. The production of extracellular proteolytic enzymes was increased when the rapA mutation was combined with a degU32 (Hy) mutation for hyper-secretion. CONCLUSIONS: Certain Rap-Phr systems of B. subtilis seem to suppress extracellular AprE production. Although this may be an important feature under natural conditions, repression of AprE production by these systems is not desirable under fermentation conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: Although the levels of aprE transcriptional increase in this study are moderate, engineering of Rap-Phr systems may be used to improve the yield of Bacillus strains that are used for the production of the extracellular protease AprE, or Bacillus strains that use of the aprE promoter for the production of a heterologous protein.
Authors: Chen-Yu Tsao; Liang Wang; Yoshifumi Hashimoto; Hyunmin Yi; John C March; Matthew P DeLisa; Thomas K Wood; James J Valdes; William E Bentley Journal: Appl Environ Microbiol Date: 2011-01-28 Impact factor: 4.792
Authors: Jolanda Neef; Fin J Milder; Danny G A M Koedijk; Marindy Klaassens; Erik C Heezius; Jos A G van Strijp; Andreas Otto; Dörte Becher; Jan Maarten van Dijl; Girbe Buist Journal: Appl Microbiol Biotechnol Date: 2015-07-10 Impact factor: 4.813
Authors: Tina N Ploss; Ewoud Reilman; Carmine G Monteferrante; Emma L Denham; Sjouke Piersma; Anja Lingner; Jari Vehmaanperä; Patrick Lorenz; Jan Maarten van Dijl Journal: Microb Cell Fact Date: 2016-03-29 Impact factor: 5.328