Literature DB >> 14959829

High-throughput expression and purification of 6xHis-tagged proteins in a 96-well format.

Jutta Drees1, Jason Smith, Frank Schäfer, Kerstin Steinert.   

Abstract

By using automation and affinity-tag technologies, analysis of the large number of ORFs generated by genome-sequencing projects is greatly accelerated. Protocols describing culture of E. coli in automation-compatible formats and subsequent micro-to large-scale automated purification of 6xHis-tagged proteins are presented.

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Year:  2004        PMID: 14959829     DOI: 10.1385/1-59259-679-7:179

Source DB:  PubMed          Journal:  Methods Mol Med        ISSN: 1543-1894


  1 in total

1.  Expression screening of integral membrane proteins from Helicobacter pylori 26695.

Authors:  Georgios Psakis; Sandra Nitschkowski; Caterina Holz; Daniel Kress; Manuel Maestre-Reyna; Julia Polaczek; Gerd Illing; Lars-Oliver Essen
Journal:  Protein Sci       Date:  2007-10-26       Impact factor: 6.725

  1 in total

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