Literature DB >> 1494033

Measurement of pH to quantify urease activity.

J M Bibby1, D W Hukins.   

Abstract

The rate of change of pH caused by the hydrolysis of urea was measured for urease solutions of 18 different concentrations. Concentrations were converted into an activity, A (measured in IU/cm3), by using a titrimetric method to assay the urease sample. For activities in the range 0.6-38 IU/cm3, A was related to the initial rate of change of pH, (dpH/dt)0, (measured in s-1) by the empirical relationship: A = 549(dpH/dt)0-1423(dpH/dt)2(0). Values of (dpH/dt)0 were sensitive to changes in the urease activity of about 0.6 IU/cm3.

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Year:  1992        PMID: 1494033     DOI: 10.1016/0165-022x(92)90017-5

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  3 in total

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