| Literature DB >> 1492927 |
M Q Li1, H G Hansma, J Vesenka, G Kelderman, P K Hansma.
Abstract
Reproducible, high-contrast, nanometer-resolution AFM images of uncoated plasmid DNA can be obtained with nanogram quantities of DNA with the help of two advances in sample preparation: (1) Heating a DNA solution at 35 degrees C for 10 to 20 minutes before deposition on mica helps separate and spread the DNA, and (2) Using 5 microliter drops of the heated DNA solution in the concentration range of 2 to 10 nanogram/microliter in contact with a specially prepared mica surface for 5 to 10 minutes gives optimal coverage with only nanograms of DNA.Entities:
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Year: 1992 PMID： 1492927 DOI： 10.1080/07391102.1992.10508671
Source DB: PubMed Journal: J Biomol Struct Dyn ISSN： 0739-1102