Literature DB >> 1491305

Collection of eggs and hatching and culturing second-stage larvae of Toxocara vitulorum in vitro.

R P Rajapakse1, V W Vasanthathilake, S Lloyd, S T Fernando.   

Abstract

Eggs of Toxocara vitulorum were harvested from the feces of infected buffalo calves and embryonated in vitro. Optimum conditions for hatch and culture of the second-stage larvae were determined. Maximum hatch of larvae occurred from eggs that were decoated by treatment with saturated Ca(OCl)2 for 16-24 min followed by treatment with CO2 and incubation at 37 C. Larvae could be cultured in RPMI-1640 medium for up to 3 mo but survived for only 3 wk in Eagle's minimum essential medium.

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Year:  1992        PMID： 1491305

Source DB:  PubMed          Journal:  J Parasitol        ISSN： 0022-3395            Impact factor:   1.276


  8 in total

1.  In vitro production of Toxocara canis excretory-secretory (TES) antigen.

Authors:  Divyamol Thomas; N Jeyathilakan; S Abdul Basith; T M A Senthilkumar
Journal:  J Parasit Dis       Date:  2014-12-20

2.  Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans.

Authors:  R D Iddawela; R P V J Rajapakse; N A N D Perera; Takeshi Agatsuma
Journal:  Korean J Parasitol       Date:  2007-03       Impact factor: 1.341

3.  The effect of serum and colostrum immunoglobulins from buffaloes infected with Toxocara vitulorum on T. vitulorum larvae in vitro and in vivo in mice.

Authors:  R P Rajapakse; S Lloyd; S T Fernando
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

4.  Development of a highly sensitive IgG-ELISA based on recombinant arginine kinase of Toxocara canis for serodiagnosis of visceral larva migrans in the murine model.

Authors:  Susiji Wickramasinghe; Lalani Yatawara; Mitsuru Nagataki; Misa Takamoto; Yoshiya Watanabe; R P V J Rajapakse; Kouji Uda; Tomohiko Suzuki; Takeshi Agatsuma
Journal:  Parasitol Res       Date:  2008-06-25       Impact factor: 2.289

5.  Periparturient reduction in buffalo of mitogen-induced lymphocyte proliferation and antibody to Toxocara vitulorum.

Authors:  P H Amerasinghe; V W Vasanthathilake; S Lloyd; S T Fernando
Journal:  Trop Anim Health Prod       Date:  1994-05       Impact factor: 1.559

6.  Antigen-induced protection against infection with Toxocara vitulorum larvae in mice.

Authors:  P H Amerasinghe; R P Rajapakse; S Lloyd; S T Fernando
Journal:  Parasitol Res       Date:  1992       Impact factor: 2.289

7.  In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products.

Authors:  Steffanie V Burk; Sriveny Dangoudoubiyam; Tammy Brewster-Barnes; Uneeda K Bryant; Daniel K Howe; Craig N Carter; Eric S Vanzant; Robert J Harmon; Kevin R Kazacos; Mary G Rossano
Journal:  Parasitol Res       Date:  2014-09-11       Impact factor: 2.289

8.  N-(4-Methoxyphenyl)Pentanamide, a Simplified Derivative of Albendazole, Displays Anthelmintic Properties against the Nematode Toxocara canis.

Authors:  Taís C Silva; Ana C Mengarda; Bruna L Lemes; Susana A Z Lescano; Dalete Christine S Souza; João Henrique G Lago; Josué de Moraes
Journal:  Microbiol Spectr       Date:  2022-07-28
  8 in total

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