| Literature DB >> 1489163 |
W C Janssen1, J A Rouwen, J J Hoffmann.
Abstract
HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7 antiserum. The flow cytometric method was evaluated by comparing the results with those obtained by the standard lymphocytotoxicity test and showed complete agreement in 107 selected patient samples.Entities:
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Year: 1992 PMID： 1489163 DOI： 10.1177/000456329202900608
Source DB: PubMed Journal: Ann Clin Biochem ISSN： 0004-5632 Impact factor: 2.057