Literature DB >> 1489096

Purification of cloned and genomic DNA by guanidine thiocyanate/isobutyl alcohol fractionation.

J E Nelson1, S A Krawetz.   

Abstract

We have developed a rapid and efficient method to purify cloned or high molecular weight eukaryotic DNA from cell lysates using guanidine thiocyanate and isobutyl alcohol. This simple two-step extraction procedure utilizes isobutyl alcohol to fractionate DNA away from cell lysates containing guanidine thiocyanate, yielding a relatively large quantity of pure DNA. The yield of DNA is approximately twice that obtained by the organic extraction method. DNA purified with this chaotropic-based protocol is suitable for a variety of applications including restriction analysis, double-stranded sequencing, PCR, subcloning, and transcription.

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Year:  1992        PMID： 1489096     DOI： 10.1016/0003-2697(92)90523-a

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

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5.  Purification of genomic DNA using heparin to remove nuclear proteins.

Authors:  C A Koller; V Kohli
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Journal:  Dev Genes Evol       Date:  2017-09-11       Impact factor: 0.900

9.  Positive and purifying selection influence the evolution of doublesex in the Anastrepha fraterculus species group.

Authors:  Iderval S Sobrinho; Reinaldo A de Brito
Journal:  PLoS One       Date:  2012-03-13       Impact factor: 3.240

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  10 in total

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