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Literature DB >> 1489093

Fluorimetric DNA assay for cell growth estimation.

Abstract

A growth assay using the fluorescent dye Hoechst 33258 has been developed which allows sensitive and rapid analysis of the DNA content of a variety of cell types including keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling. The assay can detect as few as 500 diploid human cells, and is compatible with the simultaneous detection of [3H]thymidine incorporation in the same cultures.

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Year: 1992 PMID： 1489093 DOI： 10.1016/0003-2697(92)90521-8

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

31 in total

1. Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis.

Authors: Christiana Ruhrberg; Holger Gerhardt; Matthew Golding; Rose Watson; Sofia Ioannidou; Hajime Fujisawa; Christer Betsholtz; David T Shima
Journal: Genes Dev Date: 2002-10-15 Impact factor: 11.361

2. Acetylation of core histones in response to HDAC inhibitors is diminished in mitotic HeLa cells.

Authors: Jason S Patzlaff; Edith Terrenoire; Bryan M Turner; William C Earnshaw; James R Paulson
Journal: Exp Cell Res Date: 2010-05-07 Impact factor: 3.905

3. A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation.

Authors: D A Lee; E Assoku; V Doyle
Journal: J Mater Sci Mater Med Date: 1998-01 Impact factor: 3.896

4. P16/p53 expression and telomerase activity in immortalized human dental pulp cells.

Authors: Obi Egbuniwe; Bernadine D Idowu; Juan M Funes; Andrew D Grant; Tara Renton; Lucy Di Silvio
Journal: Cell Cycle Date: 2011-11-15 Impact factor: 4.534

5. Engineering three-dimensional collagen-IKVAV matrix to mimic neural microenvironment.

Authors: Hossein Hosseinkhani; Yosuke Hiraoka; Chung-Hsing Li; Yi-Ru Chen; Dah-Shyong Yu; Po-Da Hong; Keng-Liang Ou
Journal: ACS Chem Neurosci Date: 2013-06-07 Impact factor: 4.418

6. Inhibition of checkpoint kinase 1 following gemcitabine-mediated S phase arrest results in CDC7- and CDK2-dependent replication catastrophe.

Authors: Nicholas J H Warren; Alan Eastman
Journal: J Biol Chem Date: 2018-12-20 Impact factor: 5.157

Fluorimetric DNA assay for cell growth estimation.

1. Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis.

2. Acetylation of core histones in response to HDAC inhibitors is diminished in mitotic HeLa cells.

3. A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation.

4. P16/p53 expression and telomerase activity in immortalized human dental pulp cells.

5. Engineering three-dimensional collagen-IKVAV matrix to mimic neural microenvironment.

6. Inhibition of checkpoint kinase 1 following gemcitabine-mediated S phase arrest results in CDC7- and CDK2-dependent replication catastrophe.

7. Generation of stable co-cultures of vascular cells in a honeycomb alginate scaffold.

8. New in vitro fluorimetric microtitration assays for toxicological screening of drugs.

9. A 135-kilodalton surface antigen of Mycoplasma hominis PG21 contains multiple directly repeated sequences.

10. In vitro polydeoxyribonucleotide effects on human pre-adipocytes.