Literature DB >> 1487237

Generation and characterization of radiation reduced cell hybrids and isolation of probes from the proximal short arm of the human X chromosome.

W Berger1, A Meindl, B de Leeuw, A de Roos, T J van de Pol, T Meitinger, S D van der Velde-Visser, H Achatz, A Geurts van Kessel, F P Cremers.   

Abstract

Employing a modified Goss-Harris irradiation fusion protocol, we have generated a panel of somatic cell hybrids containing various overlapping fragments of the Xcen-Xp11.4 interval. This region of the human X chromosome is known to carry genes for several hereditary eye diseases including retinitis pigmentosa (RP2), congenital stationary night blindness (CSNB-1) and Norrie disease. These hybrid cell lines were employed to isolate 17 new DNA probes by making use of the Alu polymerase chain reaction (PCR) method and subsequent cloning of the PCR products in a plasmid vector. With these probes, we have characterized two previously described microdeletions spanning the Norrie locus; these deletions have enabled us to subdivide the Xp11.4-p11.3 region into three defined intervals.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1487237     DOI： 10.1007/bf00220070

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  21 in total

1.  New method for mapping genes in human chromosomes.

Authors:  S J Goss; H Harris
Journal:  Nature       Date:  1975-06-26       Impact factor: 49.962

2.  Physical mapping of 60 DNA markers in the p21.1----q21.3 region of the human X chromosome.

Authors:  R G Lafrenière; C J Brown; V E Powers; L Carrel; K E Davies; D F Barker; H F Willard
Journal:  Genomics       Date:  1991-10       Impact factor: 5.736

3.  Rapid isolation of DNA probes within specific chromosome regions by interspersed repetitive sequence polymerase chain reaction.

Authors:  S A Ledbetter; D L Nelson; S T Warren; D H Ledbetter
Journal:  Genomics       Date:  1990-03       Impact factor: 5.736

4.  Assignment of the gene for complete X-linked congenital stationary night blindness (CSNB1) to Xp11.3.

Authors:  M A Musarella; R G Weleber; W H Murphey; R S Young; L Anson-Cartwright; M Mets; S P Kraft; R Polemeno; M Litt; R G Worton
Journal:  Genomics       Date:  1989-11       Impact factor: 5.736

5.  Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.

Authors:  D L Nelson; S A Ledbetter; L Corbo; M F Victoria; R Ramírez-Solis; T D Webster; D H Ledbetter; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

6.  Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries.

Authors:  P Lichter; T Cremer; J Borden; L Manuelidis; D C Ward
Journal:  Hum Genet       Date:  1988-11       Impact factor: 4.132

7.  "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1984-02       Impact factor: 3.365

8.  Physical fine-mapping of a deletion spanning the Norrie gene.

Authors:  P J Diergaarde; B Wieringa; E M Bleeker-Wagemakers; K B Sims; X O Breakefield; H H Ropers
Journal:  Hum Genet       Date:  1989-12       Impact factor: 4.132

9.  Toward a complete linkage map of the human X chromosome: regional assignment of 16 cloned single-copy DNA sequences employing a panel of somatic cell hybrids.

Authors:  P Wieacker; K E Davies; H J Cooke; P L Pearson; R Williamson; S Bhattacharya; J Zimmer; H H Ropers
Journal:  Am J Hum Genet       Date:  1984-03       Impact factor: 11.025

10.  Norrie disease resulting from a gene deletion: clinical features and DNA studies.

Authors:  D Donnai; R C Mountford; A P Read
Journal:  J Med Genet       Date:  1988-02       Impact factor: 6.318
View more
  2 in total

Review 1.  Irradiation and fusion gene transfer.

Authors:  M A Walter; P N Goodfellow
Journal:  Mol Biotechnol       Date:  1995-04       Impact factor: 2.695

2.  Localization of X chromosome short arm markers relative to synovial sarcoma- and renal adenocarcinoma-associated translocation breakpoints.

Authors:  R J Sinke; B de Leeuw; H A Janssen; D O Weghuis; R F Suijkerbuijk; A M Meloni; S Gilgenkrantz; W Berger; H H Ropers; A A Sandberg
Journal:  Hum Genet       Date:  1993-10-01       Impact factor: 4.132
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.