P W Rieck1, J Kriegsch, C Jaeckel, C Hartmann. 1. Augenklinik der Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Berlin. peter.rieck@charite.de
Abstract
BACKGROUND: New approaches for the treatment of secondary cataract focus on a pharmacological therapy to prevent posterior capsule opacification. In this study we investigated the effect of the naphthylurea suramin in vitro in a cell culture model. MATERIAL AND METHODS: Cell proliferation was measured using a proliferation assay and cell migration with a migration assay. Analyses of suramin toxicity were carried out using trypan blue staining. RESULTS: A significant dose-dependent effect of suramin on the inhibition of LEC proliferation and migration was found even after short (1 or 2 h) incubation times ( p<0.01). Cell lysis reflecting a cytotoxic effect of suramin was not found even with high concentrations. However, preincubation of lens capsules with suramin did not prevent LECs from migrating compared to control cultures. CONCLUSION: These results demonstrate the efficiency of suramin in vitro to inhibit migration of HLEC which can be explained by blocking growth factor-mediated effects on the cells. However, further investigations on the pharmacokinetic properties of suramin and possible side-effects on other intraocular tissues must follow.
BACKGROUND: New approaches for the treatment of secondary cataract focus on a pharmacological therapy to prevent posterior capsule opacification. In this study we investigated the effect of the naphthylurea suramin in vitro in a cell culture model. MATERIAL AND METHODS: Cell proliferation was measured using a proliferation assay and cell migration with a migration assay. Analyses of suramintoxicity were carried out using trypan blue staining. RESULTS: A significant dose-dependent effect of suramin on the inhibition of LEC proliferation and migration was found even after short (1 or 2 h) incubation times ( p<0.01). Cell lysis reflecting a cytotoxic effect of suramin was not found even with high concentrations. However, preincubation of lens capsules with suramin did not prevent LECs from migrating compared to control cultures. CONCLUSION: These results demonstrate the efficiency of suramin in vitro to inhibit migration of HLEC which can be explained by blocking growth factor-mediated effects on the cells. However, further investigations on the pharmacokinetic properties of suramin and possible side-effects on other intraocular tissues must follow.
Authors: K M Goins; J R Ortiz; S F Fulcher; J T Handa; G J Jaffe; G N Foulks; L M Cobo Journal: J Cataract Refract Surg Date: 1994-09 Impact factor: 3.351