| Literature DB >> 1486564 |
A L Hawkins1, R J Jones, B A Zehnbauer, M S Zicha, M J Collector, S J Sharkis, C A Griffin.
Abstract
The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.Entities:
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Year: 1992 PMID： 1486564 DOI： 10.1016/0165-4608(92)90345-9
Source DB: PubMed Journal: Cancer Genet Cytogenet ISSN： 0165-4608