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Literature DB >> 1483968

Basic conditions for the drug selection and transient gene expression in the cultured cell line of Bombyx mori.

K Okano¹, N Miyajima, N Takada, M Kobayashi, H Maekawa.

Abstract

We established basic conditions for transient gene expression and selection of antibiotics in the cultured cell line of silkworm, Bombyx mori, by use of the promoter of the heat shock protein (hsp70) gene of Drosophila melanogaster. The control promoter (hsp70) promoted the expression of chloramphenicol acetyltransferase (CAT) gene ligated at the downstream, dependent on the orientation of the promoter in the silkworm cell. The cell line is able to be supplied for the promoter assay of the silkworm genes. The concentration for the drug selection was determined as 0.75 mg/ml on neomycin analog, G418 (geneticin).

Entities: Chemical Gene Species

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Year: 1992 PMID： 1483968 DOI： 10.1007/bf02631067

Source DB: PubMed Journal: In Vitro Cell Dev Biol ISSN： 0883-8364

5 in total

1. Stable transformation of Drosophila Kc cells to antibiotic resistance with the bacterial neomycin resistance gene.

Authors: C Maisonhaute; G Echalier
Journal: FEBS Lett Date: 1986-03-03 Impact factor: 4.124

2. Transient expression of chimeric CAT genes injected into early embryos of the domesticated silkworm Bombyx mori.

Authors: T Tamura; T Kanda; S Takiya; K Okano; H Maekawa
Journal: Jpn J Genet Date: 1990-12

3. Transformation of cultured Drosophila melanogaster cells with a dominant selectable marker.

Authors: D C Rio; G M Rubin
Journal: Mol Cell Biol Date: 1985-08 Impact factor: 4.272

4. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

Authors: C M Gorman; L F Moffat; B H Howard
Journal: Mol Cell Biol Date: 1982-09 Impact factor: 4.272

5. Production of virus-like particles by the transposable genetic element, copia, of Drosophila melanogaster.

Authors: T Miyake; N Mae; T Shiba; S Kondo
Journal: Mol Gen Genet Date: 1987-04

5 in total

3 in total

Basic conditions for the drug selection and transient gene expression in the cultured cell line of Bombyx mori.

1. Stable transformation of Drosophila Kc cells to antibiotic resistance with the bacterial neomycin resistance gene.

2. Transient expression of chimeric CAT genes injected into early embryos of the domesticated silkworm Bombyx mori.

3. Transformation of cultured Drosophila melanogaster cells with a dominant selectable marker.

4. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

5. Production of virus-like particles by the transposable genetic element, copia, of Drosophila melanogaster.

1. Stable transformation of lepidopteran cultured cells with novel cloning method.

Review 2. Selectable genetic markers for nematode transgenesis.

3. Integration of the 5' end of the retrotransposon, R2Bm, can be complemented by homologous recombination.