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Literature DB >> 14824487

Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product.

Abstract

1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is obtained when an excess of methyl green is caused to fade by adjusting the pH to 7.5. 3. The compound formed by methyl green with DNA has the same maximum absorption at 642.5 to 645 mmicro in the pH range 3.5-7.8, whereas the free dye fades markedly above pH 5.0.

Entities: Chemical Species

Keywords: METHYL GREEN; NUCLEIC ACIDS

Mesh：

Substances：

Year: 1950 PMID： 14824487 PMCID： PMC2147252 DOI： 10.1085/jgp.34.2.147

Source DB: PubMed Journal: J Gen Physiol ISSN： 0022-1295 Impact factor: 4.086

3 in total

10 in total

Review 5. Energy transfer and binding competition between dyes used to enhance staining differentiation in metaphase chromosomes.

Authors: E Sahar; S A Latt
Journal: Chromosoma Date: 1980 Impact factor: 4.316

Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product.

1. Methyl green-pyronin; basis of selective staining of nucleic acids.

2. Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the reaction.

3. Methyl green-pyronin; stoichiometry of reaction with nucleic acids.

1. Rapid method for the detection of DNase of campylobacters.

2. On the mechanism of the methyl green-pyronin stain for nucleic acids.

Review 3. [New approaches in cytometry in the field of cancer research, general biology and pathology].

4. Inhibition of pyrimidine dimer formation in DNA by cationic molecules: role of energy transfer.

Review 5. Energy transfer and binding competition between dyes used to enhance staining differentiation in metaphase chromosomes.

6. A fast, low cost, and highly efficient fluorescent DNA labeling method using methyl green.

7. Methyl green is a substitute for distamycin A in the formation of distamycin A/DAPI C-bands.

8. [Coplanar hetero-oligobases (phthalanilides), highly effective cytostatics].

9. Simultaneous production of R-bands and either replication patterns or sister chromatid differentiation.

10. An analysis of the inactivation of the frog sperm nucleus by toluidine blue.