Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A lactococcal expression system for engineered nisins.

Literature DB >> 1482189

A lactococcal expression system for engineered nisins.

Abstract

The nisin-producing Lactococcus lactis strain FI5876 has been modified and developed for use as an expression system for engineered nisin variants. Insertional inactivation of the resident nisA gene had a polar effect on downstream genes, including those involved in nisin immunity. However, subsequent chromosomal rearrangements in this region involving a newly discovered insertion element (IS905) generated a strain that was deficient in the nisA gene product but expressed those nisin determinants necessary for prenisin maturation, secretion, and immunity. Complementation of the lesion in the nisA gene by plasmid-encoded nisA genes containing site-specific mutations resulted in the exclusive production of altered nisins containing specific amino acid substitutions.

Entities: Species

Mesh：

Substances：

Year: 1992 PMID： 1482189 PMCID： PMC183161 DOI： 10.1128/aem.58.11.3683-3693.1992

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

31 in total

1. Solution structures of nisin A and its two major degradation products determined by n.m.r.

Authors: L Y Lian; W C Chan; S D Morley; G C Roberts; B W Bycroft; D Jackson
Journal: Biochem J Date: 1992-04-15 Impact factor: 3.857

2. Nisin biosynthesis genes are encoded by a novel conjugative transposon.

Authors: N Horn; S Swindell; H Dodd; M Gasson
Journal: Mol Gen Genet Date: 1991-08

3. Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.

Authors: J W Mulders; I J Boerrigter; H S Rollema; R J Siezen; W M de Vos
Journal: Eur J Biochem Date: 1991-11-01

4. Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

Authors: S N Ho; H D Hunt; R M Horton; J K Pullen; L R Pease
Journal: Gene Date: 1989-04-15 Impact factor: 3.688

5. The aacA-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis.

Authors: D A Rouch; M E Byrne; Y C Kong; R A Skurray
Journal: J Gen Microbiol Date: 1987-11

6. Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.

Authors: Y Fujita; T Fujita; Y Miwa; J Nihashi; Y Aratani
Journal: J Biol Chem Date: 1986-10-15 Impact factor: 5.157

7. Insertion and amplification of foreign genes in the Lactococcus lactis subsp. lactis chromosome.

Authors: M C Chopin; A Chopin; A Rouault; N Galleron
Journal: Appl Environ Microbiol Date: 1989-07 Impact factor: 4.792

8. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors: S N Cohen; A C Chang; L Hsu
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

9. Characterization of the nisin gene as part of a polycistronic operon in the chromosome of Lactococcus lactis ATCC 11454.

Authors: M T Steen; Y J Chung; J N Hansen
Journal: Appl Environ Microbiol Date: 1991-04 Impact factor: 4.792

10. Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and posttranslational processing of its peptide product.

Authors: C Kaletta; K D Entian
Journal: J Bacteriol Date: 1989-03 Impact factor: 3.490

17 in total

Review 1. Immunity to lantibiotics.

Authors: P E Saris; T Immonen; M Reis; H G Sahl
Journal: Antonie Van Leeuwenhoek Date: 1996-02 Impact factor: 2.271

Review 2. Protein engineering of lantibiotics.

Authors: O P Kuipers; G Bierbaum; B Ottenwälder; H M Dodd; N Horn; J Metzger; T Kupke; V Gnau; R Bongers; P van den Bogaard; H Kosters; H S Rollema; W M de Vos; R J Siezen; G Jung; F Götz; H G Sahl; M J Gasson
Journal: Antonie Van Leeuwenhoek Date: 1996-02 Impact factor: 2.271

3. Enhanced production of pediocin PA-1 and coproduction of nisin and pediocin PA-1 by Lactococcus lactis.

Authors: N Horn; M I Martínez; J M Martínez; P E Hernández; M J Gasson; J M Rodríguez; H M Dodd
Journal: Appl Environ Microbiol Date: 1999-10 Impact factor: 4.792

4. YtjE from Lactococcus lactis IL1403 Is a C-S lyase with alpha, gamma-elimination activity toward methionine.

Authors: M Carmen Martínez-Cuesta; Carmen Peláez; John Eagles; Michael J Gasson; Teresa Requena; Sean B Hanniffy
Journal: Appl Environ Microbiol Date: 2006-07 Impact factor: 4.792

5. Characterization of IS905, a new multicopy insertion sequence identified in lactococci.

Authors: H M Dodd; N Horn; M J Gasson
Journal: J Bacteriol Date: 1994-06 Impact factor: 3.490

6. Sequence similarity of putative transposases links the maize Mutator autonomous element and a group of bacterial insertion sequences.

Authors: J A Eisen; M I Benito; V Walbot
Journal: Nucleic Acids Res Date: 1994-07-11 Impact factor: 16.971

7. Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp. cremoris MG1363.

Authors: Udo Wegmann; Mary O'Connell-Motherway; Aldert Zomer; Girbe Buist; Claire Shearman; Carlos Canchaya; Marco Ventura; Alexander Goesmann; Michael J Gasson; Oscar P Kuipers; Douwe van Sinderen; Jan Kok
Journal: J Bacteriol Date: 2007-02-16 Impact factor: 3.490