Literature DB >> 1480161

Electron microscopic visualisation of the 5S rRNA-YL3 complex from Saccharomyces cerevisiae.

K M Kyle1, G Harauz.   

Abstract

The complex comprising 5S ribosomal RNA and the ribosomal protein YL3 (5S rRNP) was isolated from yeast (Saccharomyces cerevisiae), and positively contrasted preparations were imaged by transmission electron microscopy. The overall dimensions of the 5S rRNP complex in the micrographs were 10 nm by 6 nm. Three predominant projections were selected from several hundred putative particles for digitisation and computer averaging to yield two-dimensional constructions with reproducible spatial resolutions exceeding 2 nm. The enhanced projection images were compatible with structural models of this complex based on biochemical studies.

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Year:  1992        PMID： 1480161     DOI： 10.1007/bf00230406

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  39 in total

1.  Effect of 50 S subunit proteins L5, L18 and L25 on the fluorescence of 5 S RNA-bound ethidium bromide.

Authors:  J Fuenteun; R Monier; R Garrett; M Le Bret; J B Le Pecq
Journal:  J Mol Biol       Date:  1975-04-25       Impact factor: 5.469

2.  The sequence of steps in the attachment of 5-S RNA to cores of Escherichia coli ribosomes.

Authors:  R S Yu; H G Wittmann
Journal:  Biochim Biophys Acta       Date:  1973-10-26

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Comparison of the structure of ribosomal 5S RNA from E. coli and from rat liver using X-ray scattering and dynamic light scattering.

Authors:  J J Müller; T N Zalkova; D Zirwer; R Misselwitz; K Gast; I N Serdyuk; H Welfle; G Damaschun
Journal:  Eur Biophys J       Date:  1986       Impact factor: 1.733

5.  5S RNA-protein complexes released by EDTA treatment of 60S ribosomal subunits of Tetrahymena thermophila.

Authors:  F Hayes; M F Guérin
Journal:  Biochimie       Date:  1987-09       Impact factor: 4.079

Review 6.  Structure and function of 5S and 5.8 S RNA.

Authors:  V A Erdmann
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1976

7.  The 5S RNA - protein complex from yeast: a model for the evolution and structure of the eukaryotic ribosome.

Authors:  R N Nazar; M Yaguchi; G E Willick
Journal:  Can J Biochem       Date:  1982-04

8.  Tertiary structure of the eukaryotic ribosomal 5 S RNA. Accessibility of phosphodiester bonds to ethylnitrosourea modification.

Authors:  J McDougall; R N Nazar
Journal:  J Biol Chem       Date:  1983-04-25       Impact factor: 5.157

9.  The 5S RNA binding protein from yeast (Saccharomyces cerevisiae) ribosomes. An RNA binding sequence in the carboxyl-terminal region.

Authors:  M Yaguchi; C F Rollin; C Roy; R N Nazar
Journal:  Eur J Biochem       Date:  1984-03-15

10.  The 5-S RNA binding protein from yeast (Saccharomyces cerevisiae) ribosomes. Evolution of the eukaryotic 5-S RNA binding protein.

Authors:  R N Nazar; M Yaguchi; G E Willick; C F Rollin; C Roy
Journal:  Eur J Biochem       Date:  1979-12-17
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  2 in total

1.  Ribosomal proteins of Thermomyces lanuginosus--characterisation by two-dimensional gel electrophoresis and differential disassembly.

Authors:  J Wu; D R Beniac; G Harauz
Journal:  Mol Cell Biochem       Date:  1995-02-09       Impact factor: 3.396

2.  Structure of recombinant rat UBF by electron image analysis and homology modelling.

Authors:  K J Neil; R A Ridsdale; B Rutherford; L Taylor; D E Larson; M Glibetic; L I Rothblum; G Harauz
Journal:  Nucleic Acids Res       Date:  1996-04-15       Impact factor: 16.971
  2 in total

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