Literature DB >> 1478485

Experimental conditions may affect reproducibility of the beta-galactosidase assay.

A Giacomini1, V Corich, F J Ollero, A Squartini, M P Nuti.   

Abstract

Several experimental conditions and parameters contributing to the determination of beta-galactosidase activity, as proposed in Miller's assay, were studied. Use of the absorbance correction factor and the nature and concentration of permeabilizing agents were taken into account as different experimental conditions. Reaction time, culture volume, and growth stage were investigated as equation parameters. From a quantitative point of view the results, in terms of Miller units, are markedly affected by variation in these conditions. Therefore, to ensure reproducibility it is advisable to use constant values for all the parameters.

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Year:  1992        PMID： 1478485     DOI： 10.1111/j.1574-6968.1992.tb14024.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  34 in total

1.  Regulation by overlapping promoters of the rate of synthesis and deposition into crystalline inclusions of Bacillus thuringiensis delta-endotoxins.

Authors:  M Sedlak; T Walter; A Aronson
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

2.  Surface signaling in ferric citrate transport gene induction: interaction of the FecA, FecR, and FecI regulatory proteins.

Authors:  S Enz; S Mahren; U H Stroeher; V Braun
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

3.  Control of the ferric citrate transport system of Escherichia coli: mutations in region 2.1 of the FecI extracytoplasmic-function sigma factor suppress mutations in the FecR transmembrane regulatory protein.

Authors:  A Stiefel; S Mahren; M Ochs; P T Schindler; S Enz; V Braun
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

4.  Effects of limited aeration and of the ArcAB system on intermediary pyruvate catabolism in Escherichia coli.

Authors:  S Alexeeva; B de Kort; G Sawers; K J Hellingwerf; M J de Mattos
Journal:  J Bacteriol       Date:  2000-09       Impact factor: 3.490

5.  Aspects of Marker/Reporter Stability and Selectivity in Soil Microbiology.

Authors:  V. Corich; A. Giacomini; P. Vian; E. Vendramin; M. Carlot; M. Basaglia; A. Squartini; S. Casella; M.P. Nuti
Journal:  Microb Ecol       Date:  2001-02       Impact factor: 4.552

6.  The FecI extracytoplasmic-function sigma factor of Escherichia coli interacts with the beta' subunit of RNA polymerase.

Authors:  Susanne Mahren; Volkmar Braun
Journal:  J Bacteriol       Date:  2003-03       Impact factor: 3.490

7.  Sites of interaction between the FecA and FecR signal transduction proteins of ferric citrate transport in Escherichia coli K-12.

Authors:  Sabine Enz; Heidi Brand; Claudia Orellana; Susanne Mahren; Volkmar Braun
Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

8.  Defined inactive FecA derivatives mutated in functional domains of the outer membrane transport and signaling protein of Escherichia coli K-12.

Authors:  Annette Sauter; Volkmar Braun
Journal:  J Bacteriol       Date:  2004-08       Impact factor: 3.490

9.  Escherichia coli exports cyclic AMP via TolC.

Authors:  Klaus Hantke; Karin Winkler; Joachim E Schultz
Journal:  J Bacteriol       Date:  2010-12-23       Impact factor: 3.490

10.  In vivo evidence for TonB dimerization.

Authors:  Annette Sauter; S Peter Howard; Volkmar Braun
Journal:  J Bacteriol       Date:  2003-10       Impact factor: 3.490
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