Literature DB >> 1477669

A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.

N D Borson1, W L Salo, L R Drewes.   

Abstract

We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.

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Year:  1992        PMID： 1477669     DOI： 10.1101/gr.2.2.144

Source DB:  PubMed          Journal:  PCR Methods Appl        ISSN： 1054-9803


  24 in total

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