Literature DB >> 1477660

Simplified template preparation and improved direct sequencing using Taq polymerase.

A K Srivastava1, V Montanaro, J Kere.   

Abstract

A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.

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Year:  1992        PMID： 1477660     DOI： 10.1101/gr.1.4.255

Source DB:  PubMed          Journal:  PCR Methods Appl        ISSN： 1054-9803


  7 in total

1.  mtDNA analysis shows common ancestry in two kindreds with X-linked recessive hypoparathyroidism and reveals a heteroplasmic silent mutation.

Authors:  S Mumm; M P Whyte; R V Thakker; K H Buetow; D Schlessinger
Journal:  Am J Hum Genet       Date:  1997-01       Impact factor: 11.025

2.  Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.

Authors:  Y G Liu; N Mitsukawa; R F Whittier
Journal:  Nucleic Acids Res       Date:  1993-07-11       Impact factor: 16.971

3.  A 1.5-megabase yeast artificial chromosome contig from human chromosome 10q11.2 connecting three genetic loci (RET, D10S94, and D10S102) closely linked to the MEN2A locus.

Authors:  T C Lairmore; S Dou; J R Howe; D Chi; K Carlson; R Veile; S K Mishra; S A Wells; H Donis-Keller
Journal:  Proc Natl Acad Sci U S A       Date:  1993-01-15       Impact factor: 11.205

4.  Parent-of-origin effects in multiple endocrine neoplasia type 2B.

Authors:  K M Carlson; J Bracamontes; C E Jackson; R Clark; A Lacroix; S A Wells; P J Goodfellow
Journal:  Am J Hum Genet       Date:  1994-12       Impact factor: 11.025

5.  Characterization and sequencing of the sex determining region Y gene (Sry) in Akodon (Cricetidae) species with sex reversed females.

Authors:  N O Bianchi; M S Bianchi; G Bailliet; A de la Chapelle
Journal:  Chromosoma       Date:  1993-06       Impact factor: 4.316

6.  Single missense mutation in the tyrosine kinase catalytic domain of the RET protooncogene is associated with multiple endocrine neoplasia type 2B.

Authors:  K M Carlson; S Dou; D Chi; N Scavarda; K Toshima; C E Jackson; S A Wells; P J Goodfellow; H Donis-Keller
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-15       Impact factor: 11.205

7.  Truncated erythropoietin receptor causes dominantly inherited benign human erythrocytosis.

Authors:  A de la Chapelle; A L Träskelin; E Juvonen
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-15       Impact factor: 11.205
  7 in total

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