| Literature DB >> 14770005 |
Christian Thoma1, Antje Ostareck-Lederer, Matthias W Hentze.
Abstract
In cells, the poly(A) tail stimulates translation from messenger RNAs bearing a cap structure or viral IRES elements. This 3' end-mediated stimulation of translation is not reflected in commonly used commercial cell-free translation systems prepared from rabbit reticulocytes or wheat germ. We describe a simple procedure to generate poly(A) tail-responsive translation extracts from HeLa cells. We suggest that this procedure should be adaptable to many animal cell lines.Entities:
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Year: 2004 PMID： 14770005 DOI： 10.1385/1-59259-750-5:171
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745