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Literature DB >> 14769992

Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in Saccharomyces cerevisiae.

Michael-Christopher Keogh¹, Stephen Buratowski.

Abstract

The chromatin immunoprecipitation (ChIP) technique has been used to determine where and under what conditions DNA binding proteins associate with specific DNA sequences. Proteins are crosslinked in vivo with formaldehyde, and chromatin is then isolated and sheared. The protein of interest is then immunoprecipitated and the associated DNA sequences identified via PCR. Although this technique was originally designed to assay DNA binding proteins, it can also be used to monitor mRNA processing factors associated with transcription complexes.

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Year: 2004 PMID： 14769992 DOI： 10.1385/1-59259-750-5:001

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

34 in total

10. Functional characterization of a Trypanosoma brucei TATA-binding protein-related factor points to a universal regulator of transcription in trypanosomes.

Authors: Jia-Peng Ruan; George K Arhin; Elisabetta Ullu; Christian Tschudi
Journal: Mol Cell Biol Date: 2004-11 Impact factor: 4.272

Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in Saccharomyces cerevisiae.

1. Distinct role of Mediator tail module in regulation of SAGA-dependent, TATA-containing genes in yeast.

2. Genes with internal repeats require the THO complex for transcription.

3. The cotranscriptional assembly of snoRNPs controls the biosynthesis of H/ACA snoRNAs in Saccharomyces cerevisiae.

4. Protein characterization of Saccharomyces cerevisiae RNA polymerase II after in vivo cross-linking.

5. Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination.

6. Gene loops function to maintain transcriptional memory through interaction with the nuclear pore complex.

7. Sub1 and RPA associate with RNA polymerase II at different stages of transcription.

8. H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway.

9. Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment.

10. Functional characterization of a Trypanosoma brucei TATA-binding protein-related factor points to a universal regulator of transcription in trypanosomes.