Literature DB >> 1476794

PCR-SSCP: a method for detection of mutations.

K Hayashi1.   

Abstract

PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) analysis is one of the simplest and perhaps one of the most sensitive methods for detection of mutations based on PCR technology. The principles of PCR-SSCP, guidelines for experiments, and applications of this technique in various fields are reviewed.

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Year:  1992        PMID: 1476794     DOI: 10.1016/1050-3862(92)90001-l

Source DB:  PubMed          Journal:  Genet Anal Tech Appl        ISSN: 1050-3862


  38 in total

1.  Molecular detection and identification of an enterovirus during an outbreak of aseptic meningitis.

Authors:  N Siafakas; A Georgopoulou; P Markoulatos; N Spyrou; G Stanway
Journal:  J Clin Lab Anal       Date:  2001       Impact factor: 2.352

2.  Use of single-strand conformation polymorphism analysis to examine the variability of the rpoS sequence in environmental isolates of Salmonellae.

Authors:  S J Jordan; C E Dodd; G S Stewart
Journal:  Appl Environ Microbiol       Date:  1999-08       Impact factor: 4.792

3.  Automated DNA mutation analysis by single-strand conformation polymorphism using capillary electrophoresis with laser-induced fluorescence detection.

Authors:  M S Liu; S Rampal; D Hsiang; F T Chen
Journal:  Mol Biotechnol       Date:  2000-05       Impact factor: 2.695

Review 4.  Molecular detection of antimicrobial resistance.

Authors:  A C Fluit; M R Visser; F J Schmitz
Journal:  Clin Microbiol Rev       Date:  2001-10       Impact factor: 26.132

5.  Considerations in adding mutation detection services to a sequencing core facility.

Authors:  M R Bonner; L W Ballard
Journal:  J Biomol Tech       Date:  1999-12

6.  Fluorescence-based mutation detection. Single-strand conformation polymorphism analysis (F-SSCP).

Authors:  J S Ellison
Journal:  Mol Biotechnol       Date:  1996-02       Impact factor: 2.695

7.  In vivo accumulation of the same anti-melanoma T cell clone in two different metastatic sites.

Authors:  M Hishii; D Andrews; L A Boyle; J T Wong; F Pandolfi; P J van den Elsen; J T Kurnick
Journal:  Proc Natl Acad Sci U S A       Date:  1997-02-18       Impact factor: 11.205

8.  Genetic Variation of MSP-1 Gene in Plasmodium vivax Isolated from Patients in Hormozgan Province, Iran using SSCP-PCR.

Authors:  A Miahipour; H Keshavarz; A Heidari; A Raeisi; M Rezaeian; S Rezaie
Journal:  Iran J Parasitol       Date:  2012       Impact factor: 1.012

9.  Characterization of novel CAPN3 isoforms in white blood cells: an alternative approach for limb-girdle muscular dystrophy 2A diagnosis.

Authors:  L Blázquez; M Azpitarte; A Sáenz; M Goicoechea; D Otaegui; X Ferrer; I Illa; E Gutierrez-Rivas; J J Vilchez; A López de Munain
Journal:  Neurogenetics       Date:  2008-06-19       Impact factor: 2.660

10.  Mutational analysis of serotonin receptor genes: HTR3A and HTR3B in fibromyalgia patients.

Authors:  Bernd Frank; Beate Niesler; Brigitta Bondy; Michael Späth; Dieter E Pongratz; Manfred Ackenheil; Christine Fischer; Gudrun Rappold
Journal:  Clin Rheumatol       Date:  2004-05-07       Impact factor: 2.980

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