Literature DB >> 1476737

A simple, efficient method to create a cDNA library.

W N Hu1, W Kopachik, R N Band.   

Abstract

The simplified "All In One Tube" protocol for constructing a cDNA library combines the advantages of the "Classic method" and the Okayama-Berg method while overcoming some of their drawbacks. In this method, adding adapters, linkers or enzyme digestion steps are no longer necessary after cDNA synthesis, thus making it quicker and especially useful when dealing with small samples of mRNA.

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Year:  1992        PMID: 1476737

Source DB:  PubMed          Journal:  Biotechniques        ISSN: 0736-6205            Impact factor:   1.993


  1 in total

1.  Identification of an immediate-early gene in the Marek's disease virus long internal repeat region which encodes a unique 14-kilodalton polypeptide.

Authors:  Y Hong; P M Coussens
Journal:  J Virol       Date:  1994-06       Impact factor: 5.103

  1 in total

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