| Literature DB >> 1476737 |
W N Hu1, W Kopachik, R N Band.
Abstract
The simplified "All In One Tube" protocol for constructing a cDNA library combines the advantages of the "Classic method" and the Okayama-Berg method while overcoming some of their drawbacks. In this method, adding adapters, linkers or enzyme digestion steps are no longer necessary after cDNA synthesis, thus making it quicker and especially useful when dealing with small samples of mRNA.Mesh:
Substances:
Year: 1992 PMID: 1476737
Source DB: PubMed Journal: Biotechniques ISSN: 0736-6205 Impact factor: 1.993