OBJECTIVE: To study the relationship between the polymorphism myeloperoxidase (MPO) gene and the genetic susceptibility to benzene toxicity in workers exposed to benzene and in patients with benzene poisoning. METHODS: Using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) techniques, the genotypes' polymorphism of MPO gene in 35 patients with chronic benzene poisoning, 46 workers exposed to benzene from the same workplace (as exposed control) and 26 controls were analyzed. RESULT: There were three (G/G, G/A and A/A) genotypes in the region of 463 bp upstream of MPO gene. The distribution frequency in G/G wild-type genotype in patients was 27.4% more than that in the exposed workers. The risk of benzene-hematotoxicity in those with G/G genotype was 2.8-fold higher than G/A + A/A genotype (OR = 2.835, 95% CI: 1.065 - 7.549, P < 0.05). The polymorphism of myeloperoxidase was not associated with gender specific. CONCLUSION: In the same benzene-exposed environment, the subjects with MPO-463 G/G genotype may be more susceptible to benzene toxicity.
OBJECTIVE: To study the relationship between the polymorphism myeloperoxidase (MPO) gene and the genetic susceptibility to benzenetoxicity in workers exposed to benzene and in patients with benzenepoisoning. METHODS: Using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) techniques, the genotypes' polymorphism of MPO gene in 35 patients with chronic benzenepoisoning, 46 workers exposed to benzene from the same workplace (as exposed control) and 26 controls were analyzed. RESULT: There were three (G/G, G/A and A/A) genotypes in the region of 463 bp upstream of MPO gene. The distribution frequency in G/G wild-type genotype in patients was 27.4% more than that in the exposed workers. The risk of benzene-hematotoxicity in those with G/G genotype was 2.8-fold higher than G/A + A/A genotype (OR = 2.835, 95% CI: 1.065 - 7.549, P < 0.05). The polymorphism of myeloperoxidase was not associated with gender specific. CONCLUSION: In the same benzene-exposed environment, the subjects with MPO-463 G/G genotype may be more susceptible to benzenetoxicity.
Authors: Qing Lan; Luoping Zhang; Guilan Li; Roel Vermeulen; Rona S Weinberg; Mustafa Dosemeci; Stephen M Rappaport; Min Shen; Blanche P Alter; Yongji Wu; William Kopp; Suramya Waidyanatha; Charles Rabkin; Weihong Guo; Stephen Chanock; Richard B Hayes; Martha Linet; Sungkyoon Kim; Songnian Yin; Nathaniel Rothman; Martyn T Smith Journal: Science Date: 2004-12-03 Impact factor: 47.728
Authors: Qing Lan; Luoping Zhang; Min Shen; William J Jo; Roel Vermeulen; Guilan Li; Christopher Vulpe; Sophia Lim; Xuefeng Ren; Stephen M Rappaport; Sonja I Berndt; Meredith Yeager; Jeff Yuenger; Richard B Hayes; Martha Linet; Songnian Yin; Stephen Chanock; Martyn T Smith; Nathaniel Rothman Journal: Carcinogenesis Date: 2008-10-31 Impact factor: 4.944