PURPOSE: To determine if aquaporin-1 expression is decreased in human and mouse corneal endothelial dysfunction. METHODS: Immunohistochemistry with anti-aquaporin 1 antibody and confocal microscopy were used to study a case series of human corneal specimens, and a mouse model of corneal endothelial injury was created with injection of sterile hot water into the anterior chamber with a 33 gauge needle. Western blotting of mouse and human corneas was used to provide confirmatory evidence. RESULTS: Aquaporin-1 was found to be expressed in normal human cornea and decreased in human corneas with endothelial disease but not in human corneas with non-endothelial corneal disease. Aquaporin-1 was also found to be reduced in mouse corneas subjected to corneal endothelial injury. Both results were confirmed by western blot analysis. CONCLUSIONS: Corneal endothelial injury is associated with decreased aquaporin-1 expression.
PURPOSE: To determine if aquaporin-1 expression is decreased in human and mousecorneal endothelial dysfunction. METHODS: Immunohistochemistry with anti-aquaporin 1 antibody and confocal microscopy were used to study a case series of human corneal specimens, and a mouse model of corneal endothelial injury was created with injection of sterile hot water into the anterior chamber with a 33 gauge needle. Western blotting of mouse and human corneas was used to provide confirmatory evidence. RESULTS:Aquaporin-1 was found to be expressed in normal human cornea and decreased in human corneas with endothelial disease but not in human corneas with non-endothelial corneal disease. Aquaporin-1 was also found to be reduced in mouse corneas subjected to corneal endothelial injury. Both results were confirmed by western blot analysis. CONCLUSIONS:Corneal endothelial injury is associated with decreased aquaporin-1 expression.
Authors: David P Dimasi; Kathryn P Burdon; Alex W Hewitt; Ravi Savarirayan; Paul R Healey; Paul Mitchell; David A Mackey; Jamie E Craig Journal: Mol Vis Date: 2010-03-31 Impact factor: 2.367
Authors: Melinda J Goodyear; Barbara M Junghans; Loretta Giummarra; Melanie J Murphy; David P Crewther; Sheila G Crewther Journal: Mol Vis Date: 2008-02-08 Impact factor: 2.367