Re-evaluation of the afferent connections of the pituitary in the weakly electric fish Apteronotus leptorhynchus: an in vitro tract-tracing study.

The pituitary plays a key role in the interaction between the brain and the endocrine system. We re-examined the afferent connections of the pituitary in the weakly electric fish Apteronotus leptorhynchus using the in vitro application of dextran-tetramethylrhodamine to the pituitary. The resultant retrograde labeling was analyzed. Application of the tracer to the rostral part, but not the caudal part, of the pituitary labels hypothalamic cells in the anterior division of the periventricular nucleus, the suprachiasmatic nucleus, and the nucleus tuberis lateralis pars anterior. Application of the tracer to either the rostral or caudal parts of the pituitary labels hypothalamic cells in the posterior division of the periventricular nucleus (RPPp), the nucleus hypothalamus caudalis (Hc), the nucleus hypothalamus anterioris, the ventral hypothalamic nucleus, and the central nucleus of the inferior lobe. Furthermore, cells in the rostral two-thirds of the brainstem reticular formation (RF) project to the entire rostrocaudal extent of the pituitary. The largest projections to the pituitary are from Hc, PPp, and RF. Of the cells in Hc that project to the pituitary, almost all (96%) are small and the remainder are medium-sized. Of the cells in PPp that project to the pituitary, about half are small or medium-sized (44% and 56%, respectively). In Hc and PPp, about one-third to one-half of the cells that project to the pituitary are markedly elongated. The cells in RF that project to the pituitary are small (4%), medium-sized (89%), or large (7%) and about four-fifths of these cells are markedly elongated. With regard to the RF projections, the pituitary may receive copies of motor instructions and sensory information supplied by collaterals of the descending and ascending projection systems of RF cells. Thus, the ongoing motor activity of the animal and the ensuing sensory feedback from this activity could directly influence the pituitary. Copyright 2004 Wiley-Liss, Inc.