| Literature DB >> 14753729 |
C Dall'Asta1, G Galaverna, A Dossena, R Marchelli.
Abstract
In this paper, we propose a new, rapid, highly sensitive and reproducible RP-HPLC-FLD method for the detection of ochratoxin A (OTA) in wine, by directly injecting the liquid in the chromatographic system without any extraction or clean-up. An alkaline mobile phase (NH4Cl:CH-CN 85:15 (v/v), 20 mM, pH 9.8) was used to obtain a distinct fluorescence enhancement. This improvement allows to reach, without an immunoaffinity clean-up or concentration, a detection limit of 0.05 ng/ml, which is similar to those commonly obtained after immunoaffinity purification and acidic elution. The method was statistically validated and directly applied to a series of wine samples.Entities:
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Year: 2004 PMID： 14753729 DOI： 10.1016/j.chroma.2003.10.025
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759