Stefanie Pabst1, Kathrin Spekl, Wolfgang Dörr. 1. Klinik und Poliklinik für Strahlentherapie und Radioonkologie, Medizinische Fakultät Carl Gustav Carus, Technischen Universität, Fetscherstrasse 74, D-01307 Dresden, Germany.
Abstract
PURPOSE: The aim of the present study was to quantify the fractionation effect in mouse oral mucosa during a daily fractionated protocol. METHODS AND MATERIALS: Irradiation of the snout of C3H mice was performed with 5 x 3 Gy/week. In the first experiment, graded test doses were applied to the lower tongue on Days 4, 7, 11, 14, or 18. Subsequently, a split-dose experiment was performed on the same days with 1, 2, 3, or 5 fractions (graded doses). RESULTS: The ED50 (dose expected to induce ulcer in 50% of the mice) for untreated mucosa was 10.7 +/- 1.0 Gy. Fractionated irradiation with 5 x 3 Gy/week yielded an ED50 for top-up irradiation of 6.5 +/- 1.8 Gy, 6.8 +/- 0.9 Gy, 5.3 +/- 2.1 Gy, 7.3 +/- 1.9 Gy, and 7.5 +/- 1.3 Gy on Days 4, 7, 11, 14, and 18. The ED50 values for split-dose irradiation on Day 4 increased from 6.24 +/- 1.48 Gy (1 fraction) to 9.96 +/- 1.47 Gy (5 fractions). Similarly, an increase from 4.04 +/- 1.56 Gy to 10.07 +/- 1.9 Gy was found on Day 11, and from 5.84 +/- 2.37 Gy to 9.81 +/- 2.29 Gy on Day 18. After the weekend breaks, values between 6.22 +/- 1.60 Gy (1 fraction) and 4.7 +/- 1.95 Gy (5 fractions) were observed on Day 7, and between 5.62 +/- 1.36 Gy and 5.98 +/- 2.01 Gy on Day 14. CONCLUSIONS: These results indicate that the fractionation effect in oral mucosa is consistently lost over the weekends and restored during the treatment weeks.
PURPOSE: The aim of the present study was to quantify the fractionation effect in mouse oral mucosa during a daily fractionated protocol. METHODS AND MATERIALS: Irradiation of the snout of C3H mice was performed with 5 x 3 Gy/week. In the first experiment, graded test doses were applied to the lower tongue on Days 4, 7, 11, 14, or 18. Subsequently, a split-dose experiment was performed on the same days with 1, 2, 3, or 5 fractions (graded doses). RESULTS: The ED50 (dose expected to induce ulcer in 50% of the mice) for untreated mucosa was 10.7 +/- 1.0 Gy. Fractionated irradiation with 5 x 3 Gy/week yielded an ED50 for top-up irradiation of 6.5 +/- 1.8 Gy, 6.8 +/- 0.9 Gy, 5.3 +/- 2.1 Gy, 7.3 +/- 1.9 Gy, and 7.5 +/- 1.3 Gy on Days 4, 7, 11, 14, and 18. The ED50 values for split-dose irradiation on Day 4 increased from 6.24 +/- 1.48 Gy (1 fraction) to 9.96 +/- 1.47 Gy (5 fractions). Similarly, an increase from 4.04 +/- 1.56 Gy to 10.07 +/- 1.9 Gy was found on Day 11, and from 5.84 +/- 2.37 Gyto 9.81 +/- 2.29 Gy on Day 18. After the weekend breaks, values between 6.22 +/- 1.60 Gy (1 fraction) and 4.7 +/- 1.95 Gy (5 fractions) were observed on Day 7, and between 5.62 +/- 1.36 Gy and 5.98 +/- 2.01 Gy on Day 14. CONCLUSIONS: These results indicate that the fractionation effect in oral mucosa is consistently lost over the weekends and restored during the treatment weeks.
Authors: Igor Shuryak; Philip Hahnfeldt; Lynn Hlatky; Rainer K Sachs; David J Brenner Journal: Radiat Environ Biophys Date: 2009-06-18 Impact factor: 1.925