Literature DB >> 1474964

Purification and characterization of cell wall proteins of Listeria monocytogenes.

Y F Belyi1, I S Tartakovskii, S V Prosorovskii.   

Abstract

Two proteins were purified from Listeria monocytogenes cell wall using detergent extraction, Superose 6 gel chromatography. Mono Q cation-exchange chromatography and Superose 12 gel chromatography. Proteins were shown to form complexes of ca. 300 kDa and pI 4.7. These complexes could not be dissociated in 6 M urea, however, during SDS-PAGE 79 and 39 kDa monomers were formed. Immunoblot analysis showed that the proteins under investigation were common to all listerial strains tested, but were absent in strains of other bacteria. We propose that the proteins investigated here could serve as immunoserological markers for Listeria.

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Year:  1992        PMID： 1474964     DOI： 10.1007/bf00198848

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN： 0300-8584            Impact factor:   3.402


  23 in total

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Authors:  C P Timmerman; A Fleer; J M Besnier; L De Graaf; F Cremers; J Verhoef
Journal:  Infect Immun       Date:  1991-11       Impact factor: 3.441

2.  Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is co-ordinately expressed with listeriolysin in Listeria monocytogenes.

Authors:  M Leimeister-Wächter; E Domann; T Chakraborty
Journal:  Mol Microbiol       Date:  1991-02       Impact factor: 3.501

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Authors:  F Fiedler
Journal:  Infection       Date:  1988       Impact factor: 3.553

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Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

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Journal:  Bacteriol Rev       Date:  1966-06

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Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 7.  Recent developments in the study of virulence in Listeria monocytogenes.

Authors:  T Chakraborty; W Goebel
Journal:  Curr Top Microbiol Immunol       Date:  1988       Impact factor: 4.291

8.  Purification, characterization, and toxicity of the sulfhydryl-activated hemolysin listeriolysin O from Listeria monocytogenes.

Authors:  C Geoffroy; J L Gaillard; J E Alouf; P Berche
Journal:  Infect Immun       Date:  1987-07       Impact factor: 3.441

9.  Species-specific detection of Listeria monocytogenes by DNA amplification.

Authors:  H G Deneer; I Boychuk
Journal:  Appl Environ Microbiol       Date:  1991-02       Impact factor: 4.792

10.  Complement component C3 fixes selectively to the major outer membrane protein (MOMP) of Legionella pneumophila and mediates phagocytosis of liposome-MOMP complexes by human monocytes.

Authors:  C Bellinger-Kawahara; M A Horwitz
Journal:  J Exp Med       Date:  1990-10-01       Impact factor: 14.307
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  4 in total

1.  A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.

Authors:  M J Loessner; A Schneider; S Scherer
Journal:  Appl Environ Microbiol       Date:  1995-03       Impact factor: 4.792

2.  A simple colony-blot method for identification of Listeria in food samples.

Authors:  Y F Belyi; N A Varfolomeeva; I S Tartakovskii
Journal:  Med Microbiol Immunol       Date:  1995-10       Impact factor: 3.402

3.  Purification and characterization of a 58-kDa cell wall-associated protein from Listeria monocytogenes.

Authors:  Y F Belyi; I S Tartakovskii; S V Prosorovskii
Journal:  Med Microbiol Immunol       Date:  1993-05       Impact factor: 3.402

4.  Close packing of Listeria monocytogenes ActA, a natively unfolded protein, enhances F-actin assembly without dimerization.

Authors:  Matthew J Footer; John K Lyo; Julie A Theriot
Journal:  J Biol Chem       Date:  2008-06-23       Impact factor: 5.157
  4 in total

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