BACKGROUND: In this study, we wished to determine whether chondrocyte death occurs after intra-articular fracture and might thus contribute to the development of posttraumatic arthritis. METHODS: Articular fracture fragments were obtained from 30 patients and the terminal deoxynucleotide transferase-mediated dUTP nick-end labeling assay was used to identify dead or dying cells. RESULTS: Dead or dying cells were identified in all fracture fragments, with an average rate of cell death of 35%. Rates of cell death greater than 90% were seen in 4 of the 30 specimens studied. CONCLUSION: Although rates of apoptosis in osteoarthritis have been reported to average 15%, the rate in this fracture population was more than twice this value (35%). The high rate of cell death noted here may help explain the occurrence of posttraumatic arthritis even in anatomically fixed intra-articular fractures.
BACKGROUND: In this study, we wished to determine whether chondrocyte death occurs after intra-articular fracture and might thus contribute to the development of posttraumatic arthritis. METHODS: Articular fracture fragments were obtained from 30 patients and the terminal deoxynucleotide transferase-mediated dUTP nick-end labeling assay was used to identify dead or dying cells. RESULTS: Dead or dying cells were identified in all fracture fragments, with an average rate of cell death of 35%. Rates of cell death greater than 90% were seen in 4 of the 30 specimens studied. CONCLUSION: Although rates of apoptosis in osteoarthritis have been reported to average 15%, the rate in this fracture population was more than twice this value (35%). The high rate of cell death noted here may help explain the occurrence of posttraumatic arthritis even in anatomically fixed intra-articular fractures.
Authors: Josef A Stolberg-Stolberg; Bridgette D Furman; N William Garrigues; Jaewoo Lee; David S Pisetsky; Nancy A Stearns; Louis E DeFrate; Farshid Guilak; Steven A Olson Journal: J Orthop Res Date: 2013-04-25 Impact factor: 3.494
Authors: J S Lewis; W C Hembree; B D Furman; L Tippets; D Cattel; J L Huebner; D Little; L E DeFrate; V B Kraus; F Guilak; S A Olson Journal: Osteoarthritis Cartilage Date: 2011-05-12 Impact factor: 6.576
Authors: Yuki Tochigi; Joseph A Buckwalter; James A Martin; Stephen L Hillis; Peng Zhang; Tanawat Vaseenon; Abigail D Lehman; Thomas D Brown Journal: J Bone Joint Surg Am Date: 2011-03-16 Impact factor: 5.284
Authors: Jonathon D Backus; Bridgette D Furman; Troy Swimmer; Collin L Kent; Amy L McNulty; Louis E Defrate; Farshid Guilak; Steven A Olson Journal: J Orthop Res Date: 2010-11-04 Impact factor: 3.494
Authors: Y Tochigi; P Zhang; M J Rudert; T E Baer; J A Martin; S L Hillis; T D Brown Journal: Osteoarthritis Cartilage Date: 2012-10-13 Impact factor: 6.576