| Literature DB >> 14746982 |
Itabajara da Silva Vaz1, Tiago Torino Lermen, André Michelon, Carlos Alexandre Sanchez Ferreira, Daniela Reis Joaquim de Freitas, Carlos Termignoni, Aoi Masuda.
Abstract
In the present study, we report the effect of several acaricides on the enzyme activity of a Boophilus microplus recombinant glutathione S-transferase (rGST). GST was expressed in Escherichia coli and was purified with glutathione (GSH) affinity column chromatography. The kinetic constants were determined by reacting GST with the substrates 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione. We report the effect of several acaricides on the enzyme activity of rGST. Some acaricides (ethion, amitraz, chlorpyrifos, DDT, cypermethrin, diazinon, ivermectin, deltamethrin and flumethrin) inhibited rGST. Contrarily, coumaphos had an activating effect. Although the accurate mechanisms of the B. microplus resistance to acaricides remain elusive, this work helps in understanding how acaricides can interact with GST.Entities:
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Year: 2004 PMID： 14746982 DOI： 10.1016/j.vetpar.2003.11.004
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738