| Literature DB >> 1474131 |
M A Romero Rodriguez1, M L Vazquez Oderiz, J Lopez Hernandez, J Simal Lozano.
Abstract
Liquid chromatographic methodology for determination of vitamin C and organic acids in different fruits (whortleberry, blackberry, red currant, black currant, raspberry, babaco, feijoa, kiwano, passion fruit, red and yellow tamarillos, medlar, and persimmon) cultivated in Galicia, Spain are developed. Both methods use a C18 column and an RP-18 precolumn for the stationary phase. High-performance-liquid-chromatographic-(HPLC-) grade water is brought to pH 2.2 with sulphuric acid for the mobile phase. The flow rate is 0.4 mL/min, and UV detection is at 254 nm for vitamin C and 214 nm for organic acids. Coefficients of variation of 1.84, 1.20, 1.00, and 2.60% are obtained for the vitamin C, quinic acid, malic acid and citric acid, respectively.Entities:
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Year: 1992 PMID： 1474131 DOI： 10.1093/chromsci/30.11.433
Source DB: PubMed Journal: J Chromatogr Sci ISSN： 0021-9665 Impact factor: 1.618