OBJECTIVES: Acrylamide (AA) (CAS No 79-06-1) has most recently been identified as a food-borne toxicant generated during the heating process of starch-containing foods. It was the aim of the present study to investigate the trans-placental exposure of newborn infants to this possible human carcinogen by analysis of the specific haemoglobin adduct of AA ( N-2-carbamoylethylvaline, AAV) in the blood of mothers and the corresponding umbilical cord blood of neonates as a parameter of biochemical effects. METHODS: We investigated the blood of 11 women advanced in pregnancy (one smoker, ten non-smokers) and the corresponding umbilical cord blood of neonates for the N-terminal haemoglobin adducts of AA (AAV) and the smoking-specific adduct of acrylonitrile (CAS No 107-13-1) ( N-cyanoethylvaline, CEV). The limit of detection (LOD) was 5 pmol/g globin for AAV and 4 pmol/g globin for CEV. RESULTS: AAV could be determined in all blood samples of the mothers (median 21 pmol/g globin, range 18-104 pmol/g globin) as well as in the umbilical cord blood of neonates (median 10 pmol/g globin, range 6-43 pmol/g globin). The highest values were detected in the blood of the smoking mother and her child. CEV was detected only in the blood of the smoking mother (185 pmol/g globin) and the corresponding umbilical cord blood (69 pmol/g globin). DISCUSSION: AAV adduct levels in non-smoking mothers and neonates showed a good correlation (r=0.859). The concentration of AA adducts in the blood of neonates is approximately 50% of the adduct level found in the blood of the mother. In view of the shorter life span of neonatal erythrocytes and the lower body weight of newborn infants, the relative internal dose of AA in neonates (in microgrammes per kilogramme body weight) must be assumed to be at least equal to that of the mother. Because of the high cell-replication rates during foetal development, trans-placental exposure of neonates to AA might raise concerns. Neonates of smoking mothers take up much higher doses of AA than those of non-smoking mothers.
OBJECTIVES:Acrylamide (AA) (CAS No 79-06-1) has most recently been identified as a food-borne toxicant generated during the heating process of starch-containing foods. It was the aim of the present study to investigate the trans-placental exposure of newborn infants to this possible human carcinogen by analysis of the specific haemoglobin adduct of AA ( N-2-carbamoylethylvaline, AAV) in the blood of mothers and the corresponding umbilical cord blood of neonates as a parameter of biochemical effects. METHODS: We investigated the blood of 11 women advanced in pregnancy (one smoker, ten non-smokers) and the corresponding umbilical cord blood of neonates for the N-terminal haemoglobin adducts of AA (AAV) and the smoking-specific adduct of acrylonitrile (CAS No 107-13-1) ( N-cyanoethylvaline, CEV). The limit of detection (LOD) was 5 pmol/g globin for AAV and 4 pmol/g globin for CEV. RESULTS:AAV could be determined in all blood samples of the mothers (median 21 pmol/g globin, range 18-104 pmol/g globin) as well as in the umbilical cord blood of neonates (median 10 pmol/g globin, range 6-43 pmol/g globin). The highest values were detected in the blood of the smoking mother and her child. CEV was detected only in the blood of the smoking mother (185 pmol/g globin) and the corresponding umbilical cord blood (69 pmol/g globin). DISCUSSION: AAV adduct levels in non-smoking mothers and neonates showed a good correlation (r=0.859). The concentration of AA adducts in the blood of neonates is approximately 50% of the adduct level found in the blood of the mother. In view of the shorter life span of neonatal erythrocytes and the lower body weight of newborn infants, the relative internal dose of AA in neonates (in microgrammes per kilogramme body weight) must be assumed to be at least equal to that of the mother. Because of the high cell-replication rates during foetal development, trans-placental exposure of neonates to AA might raise concerns. Neonates of smoking mothers take up much higher doses of AA than those of non-smoking mothers.
Authors: Gonçalo Gamboa da Costa; Mona I Churchwell; L Patrice Hamilton; Linda S Von Tungeln; Frederick A Beland; M Matilde Marques; Daniel R Doerge Journal: Chem Res Toxicol Date: 2003-10 Impact factor: 3.739
Authors: M Schwenk; U Gundert-Remy; G Heinemeyer; K Olejniczak; R Stahlmann; W Kaufmann; H M Bolt; H Greim; E von Keutz; H P Gelbke Journal: Arch Toxicol Date: 2002-11-22 Impact factor: 5.153
Authors: Clara Amalie Gade Timmermann; Signe Sonne Mølck; Manik Kadawathagedara; Anne Ahrendt Bjerregaard; Margareta Törnqvist; Anne Lise Brantsæter; Marie Pedersen Journal: Toxics Date: 2021-06-30
Authors: Marie Pedersen; Hans von Stedingk; Maria Botsivali; Silvia Agramunt; Jan Alexander; Gunnar Brunborg; Leda Chatzi; Sarah Fleming; Eleni Fthenou; Berit Granum; Kristine B Gutzkow; Laura J Hardie; Lisbeth E Knudsen; Soterios A Kyrtopoulos; Michelle A Mendez; Domenico F Merlo; Jeanette K Nielsen; Per Rydberg; Dan Segerbäck; Jordi Sunyer; John Wright; Margareta Törnqvist; Jos C Kleinjans; Manolis Kogevinas Journal: Environ Health Perspect Date: 2012-09-23 Impact factor: 9.031
Authors: Talita Duarte-Salles; Hans von Stedingk; Berit Granum; Kristine B Gützkow; Per Rydberg; Margareta Törnqvist; Michelle A Mendez; Gunnar Brunborg; Anne Lise Brantsæter; Helle Margrete Meltzer; Jan Alexander; Margaretha Haugen Journal: Environ Health Perspect Date: 2012-11-29 Impact factor: 9.031