Literature DB >> 14739020

PCR-RFLP to identify Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis causing American cutaneous leishmaniasis.

Angela C Volpini1, Valéria M A Passos, Guilherme Correa Oliveira, Alvaro J Romanha.   

Abstract

A PCR-RFLP based method was developed to diagnose and identify the Leishmania species causing American cutaneous leishmaniasis (ACL) in a panel of clinical samples obtained from an endemic region of Brazil. The comparison of the results obtained by PCR-RFLP and PCR-hybridization in the identification of Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis were highly concordant (kappa=91.5%). The PCR-RFLP method was reliable, fast and easy to conduct on biopsies and presents potential value of utmost importance for the diagnosis and identification of Leishmania in clinical specimens, infected reservoirs and vectors.

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Year:  2004        PMID： 14739020     DOI： 10.1016/j.actatropica.2003.10.008

Source DB:  PubMed          Journal:  Acta Trop        ISSN： 0001-706X            Impact factor:   3.112


  31 in total

1.  Molecular biological identification of monoxenous trypanosomatids and Leishmania from antropophilic sand flies (Diptera: Psychodidae) in Southeast Brazil.

Authors:  Leonardo de Souza Rocha; Claudiney Biral dos Santos; Aloísio Falqueto; Gabriel Grimaldi; Elisa Cupolillo
Journal:  Parasitol Res       Date:  2010-05-25       Impact factor: 2.289

2.  Autoscreening of restriction endonucleases for PCR-restriction fragment length polymorphism identification of fungal species, with Pleurotus spp. as an example.

Authors:  Zhi-Hui Yang; Ji-Xiang Huang; Yi-Jian Yao
Journal:  Appl Environ Microbiol       Date:  2007-10-26       Impact factor: 4.792

3.  Performance of two immunochromatographic tests for diagnosis of visceral leishmaniasis in patients coinfected with HIV.

Authors:  Mauro Roberto Biá da Silva; Natália Alberto Alves Brandão; Marco Colovati; Margella Marconcine Pinheiro de Sousa; Larissa Coelho de Lima; Miriam Leandro Dorta; Fátima Ribeiro-Dias; Dorcas Lamounier Costa; Carlos Henrique Nery Costa; Milton Adriano Pelli de Oliveira
Journal:  Parasitol Res       Date:  2017-12-21       Impact factor: 2.289

4.  Characterization of Leishmania species from Central-West Region of Brazil.

Authors:  Ludiele Souza Castro; Adriana de Oliveira França; Eduardo de Castro Ferreira; Manoel Sebastião da Costa Lima Júnior; Célia Maria Ferreira Gontijo; Agnes Antônia Sampaio Pereira; Maria Elizabeth Cavalheiros Dorval
Journal:  Parasitol Res       Date:  2018-04-20       Impact factor: 2.289

5.  Leishmania sp. identification by PCR associated with sequencing of target SSU rDNA in paraffin-embedded skin samples stored for more than 30 years.

Authors:  Ana Carolina S de Lima; Ricardo A Zampieri; Thaíse Y Tomokane; Márcia D Laurenti; Fernando T Silveira; Carlos E P Corbett; Lucile M Floeter-Winter; Cláudia M C Gomes
Journal:  Parasitol Res       Date:  2010-12-15       Impact factor: 2.289

Review 6.  Species typing in dermal leishmaniasis.

Authors:  Gert Van der Auwera; Jean-Claude Dujardin
Journal:  Clin Microbiol Rev       Date:  2015-04       Impact factor: 26.132

7.  Evaluation of a pan-Leishmania SL RNA qPCR assay for parasite detection in laboratory-reared and field-collected sand flies and reservoir hosts.

Authors:  Myrthe Pareyn; Rik Hendrickx; Nigatu Girma; Sarah Hendrickx; Lieselotte Van Bockstal; Natalie Van Houtte; Simon Shibru; Louis Maes; Herwig Leirs; Guy Caljon
Journal:  Parasit Vectors       Date:  2020-06-01       Impact factor: 3.876

8.  Leishmania infantum AS A CAUSATIVE AGENT OF CUTANEOUS LEISHMANIASIS IN THE STATE OF MATO GROSSO DO SUL, BRAZIL.

Authors:  Ludiele Souza Castro; Adriana de Oliveira França; Eduardo de Castro Ferreira; Günther Hans Filho; Minoru German Higa Júnior; Célia Maria Ferreira Gontijo; Agnes Antônia Sampaio Pereira; Maria Elizabeth Moraes C Dorval
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2016-03-22       Impact factor: 1.846

9.  A FRET-based real-time PCR assay to identify the main causal agents of New World tegumentary leishmaniasis.

Authors:  Pablo Tsukayama; Jorge H Núñez; Maxy De Los Santos; Valeria Soberón; Carmen M Lucas; Greg Matlashewski; Alejandro Llanos-Cuentas; Marianela Ore; G Christian Baldeviano; Kimberly A Edgel; Andres G Lescano; Paul C F Graf; David J Bacon
Journal:  PLoS Negl Trop Dis       Date:  2013-01-03

10.  Case Report: Atypical Cutaneous Leishmaniasis in a Patient with Mixed Leishmania guyanensis and Leishmania amazonensis Infection.

Authors:  Carina Scolari Gosch; Bruna Silva Resende; Célia Bastos Amorim; Cálita Pollyanna Marques; Ledice Inacia de Araújo Pereira; Sebastião Alves Pinto; Silvia Reni Bortolin Uliana; Adriano Cappellazzo Coelho; Fátima Ribeiro-Dias; Miriam Leandro Dorta
Journal:  Am J Trop Med Hyg       Date:  2018-11       Impact factor: 2.345
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