BACKGROUND: Diabetic nephropathy (DN) is associated with functional changes in the filtration barrier, and microalbuminuria is a strong predictor of the development of overt DN. Nephrin is a novel podocyte-specific protein which localizes at the slit diaphragm. This study examines the expression of nephrin mRNA in the kidneys of type 2 diabetics with DN. METHODS: Renal tissues were obtained from 13 type 2 diabetics with DN. We also examined samples from five patients with minimal change nephrotic syndrome (MCNS) and five normal kidneys (normals) as control. The severity of DN was classified into two grades based on histopathological findings. DN grade 1 (DN1 = seven patients) presented mild mesangial expansion, and DN grade 2 (DN2 = six patients) moderate mesangial expansion. Nephrin mRNA was quantitated and localized by in situ hybridization. RESULTS: Cells positive for nephrin mRNA were detected exclusively in glomerular epithelial cells. The percentage of cells positive for nephrin mRNA in DN2 was significantly lower than in MCNS and normal kidneys. Furthermore, there was an inverse correlation between the percentage of cells positive for nephrin mRNA and extent of proteinuria. CONCLUSION: The low expression of nephrin mRNA may be closely linked to development and/or progression of proteinuria in human diabetic nephropathy.
BACKGROUND:Diabetic nephropathy (DN) is associated with functional changes in the filtration barrier, and microalbuminuria is a strong predictor of the development of overt DN. Nephrin is a novel podocyte-specific protein which localizes at the slit diaphragm. This study examines the expression of nephrin mRNA in the kidneys of type 2 diabetics with DN. METHODS: Renal tissues were obtained from 13 type 2 diabetics with DN. We also examined samples from five patients with minimal change nephrotic syndrome (MCNS) and five normal kidneys (normals) as control. The severity of DN was classified into two grades based on histopathological findings. DN grade 1 (DN1 = seven patients) presented mild mesangial expansion, and DN grade 2 (DN2 = six patients) moderate mesangial expansion. Nephrin mRNA was quantitated and localized by in situ hybridization. RESULTS: Cells positive for nephrin mRNA were detected exclusively in glomerular epithelial cells. The percentage of cells positive for nephrin mRNA in DN2 was significantly lower than in MCNS and normal kidneys. Furthermore, there was an inverse correlation between the percentage of cells positive for nephrin mRNA and extent of proteinuria. CONCLUSION: The low expression of nephrin mRNA may be closely linked to development and/or progression of proteinuria in humandiabetic nephropathy.
Authors: Xuezhu Li; Peter Y Chuang; Vivette D D'Agati; Yan Dai; Rabi Yacoub; Jia Fu; Jin Xu; Oltjon Taku; Prem K Premsrirut; Lawrence B Holzman; John Cijiang He Journal: J Am Soc Nephrol Date: 2015-02-02 Impact factor: 10.121
Authors: Clemens D Cohen; Andreas Klingenhoff; Anissa Boucherot; Almut Nitsche; Anna Henger; Bodo Brunner; Holger Schmid; Monika Merkle; Moin A Saleem; Klaus-Peter Koller; Thomas Werner; Hermann-Josef Gröne; Peter J Nelson; Matthias Kretzler Journal: Proc Natl Acad Sci U S A Date: 2006-03-31 Impact factor: 11.205
Authors: Tamara Turk; Jan Willem Leeuwis; Julia Gray; Suzy V Torti; Karen M Lyons; Tri Q Nguyen; Roel Goldschmeding Journal: J Histochem Cytochem Date: 2009-03-02 Impact factor: 2.479
Authors: Mervi Ristola; Satu Arpiainen; Moin A Saleem; Peter W Mathieson; Gavin I Welsh; Sanna Lehtonen; Harry Holthöfer Journal: BMC Mol Biol Date: 2009-08-24 Impact factor: 2.946