| Literature DB >> 14732230 |
Joo Heon Kim1, Sun Young Yoon, Chang-Nam Kim, Joung Hyuck Joo, Sang Kyoung Moon, In Seong Choe, Yong-Kyung Choe, Jae Wha Kim.
Abstract
To clarify the roles of Bmi-1 in colorectal carcinoma, we examined the expression of Bmi-1 in 41 samples out of 46 colorectal carcinomas by reverse transcription-PCR, whereas all 46 were analyzed by immunostaining. In addition, we analyzed the expression patterns of Bmi-1 in association with p16INK4a and p14ARF (in mouse p19ARF) in a series of colorectal carcinomas. The level of Bmi-1 mRNA in the carcinoma tissues was significantly higher than those of the adjacent non-neoplastic colonic mucosal tissues. Immunohistochemistry for Bmi-1 showed moderate or strong expression levels in 65% (30/46) of colorectal carcinomas. Colorectal carcinomas with moderate or strong Bmi-1 expression were more likely to have low levels of the INK4 locus proteins (p16INK4a/p14ARF) (P<0.07). These results suggested that modulation of Bmi-1 protein might be involved in human colorectal carcinogenesis by repressing the INK4a/ARF proteins.Entities:
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Year: 2004 PMID： 14732230 DOI： 10.1016/j.canlet.2003.07.009
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679