OBJECTIVE: In the arterial response to injury, collagen breakdown has been studied extensively, but little is known on collagen synthesis and fiber formation. Here, we studied in vivo collagen synthesis and collagen fiber content in relation to collagen breakdown following arterial balloon injury. METHODS AND RESULTS: Twenty-five New Zealand White rabbits were balloon dilated in femoral and iliac arteries and terminated at 2, 7, 14 and 28 days. From day 7, both constrictive arterial remodeling and intimal hyperplasia were observed. Collagen degradation, synthesis and fiber content were studied using zymography, quantitative Polymerase Chain Reaction, Western blotting and picrosirius red staining. Collagen synthesis, reflected by procollagen I and heat shock protein 47 (Hsp47) expression, increased at day 2 with a maximum at day 14 and was accompanied by increased collagen breakdown as reflected by matrix metalloproteinase-1 and -2 levels. Collagen content in media and adventitia only increased between days 2 and 7 after balloon injury. CONCLUSIONS: In the first week after arterial injury, increased collagen content is associated with increased collagen synthesis and degradation. However, after 1 week, collagen turnover remains high in contrast to increased collagen fiber content. This suggests that after 1 week, collagen turnover is used for other processes like cell migration and arterial remodeling.
OBJECTIVE: In the arterial response to injury, collagen breakdown has been studied extensively, but little is known on collagen synthesis and fiber formation. Here, we studied in vivo collagen synthesis and collagen fiber content in relation to collagen breakdown following arterial balloon injury. METHODS AND RESULTS: Twenty-five New Zealand White rabbits were balloon dilated in femoral and iliac arteries and terminated at 2, 7, 14 and 28 days. From day 7, both constrictive arterial remodeling and intimal hyperplasia were observed. Collagen degradation, synthesis and fiber content were studied using zymography, quantitative Polymerase Chain Reaction, Western blotting and picrosirius red staining. Collagen synthesis, reflected by procollagen I and heat shock protein 47 (Hsp47) expression, increased at day 2 with a maximum at day 14 and was accompanied by increased collagen breakdown as reflected by matrix metalloproteinase-1 and -2 levels. Collagen content in media and adventitia only increased between days 2 and 7 after balloon injury. CONCLUSIONS: In the first week after arterial injury, increased collagen content is associated with increased collagen synthesis and degradation. However, after 1 week, collagen turnover remains high in contrast to increased collagen fiber content. This suggests that after 1 week, collagen turnover is used for other processes like cell migration and arterial remodeling.
Authors: Nima Etminan; Rita Dreier; Bruce A Buchholz; Kerim Beseoglu; Peter Bruckner; Christian Matzenauer; James C Torner; Robert D Brown; Hans-Jakob Steiger; Daniel Hänggi; R Loch Macdonald Journal: Stroke Date: 2014-04-29 Impact factor: 7.914
Authors: Jia Liu; Alain van Mil; Krijn Vrijsen; Jiajun Zhao; Ling Gao; Corina H G Metz; Marie-José Goumans; Pieter A Doevendans; Joost P G Sluijter Journal: J Cell Mol Med Date: 2010-06-09 Impact factor: 5.310
Authors: Sebastian Grundmann; Stephan H Schirmer; Liesbeth H P Hekking; Jan Andries Post; Mihaela G Ionita; Daphne de Groot; Niels van Royen; Bernard van den Berg; Hans Vink; Martin Moser; Christoph Bode; Dominique de Kleijn; Gerard Pasterkamp; Jan J Piek; Imo E Hoefer Journal: J Cell Mol Med Date: 2009-03-06 Impact factor: 5.310
Authors: T I G van der Spoel; W A Gathier; S Koudstaal; F van Slochteren; S Jansen Of Lorkeers; J P G Sluijter; I E Hoefer; P Steendijk; M J M Cramer; P A Doevendans; E van Belle; S A J Chamuleau Journal: J Cardiovasc Transl Res Date: 2015-09-17 Impact factor: 4.132