| Literature DB >> 14727812 |
Ena Nakatsukasa1, Naomi Kashiwazaki, Akiko Takizawa, Masao Shino, Kazuhiro Kitada, Tadao Serikawa, Yoji Hakamata, Eiji Kobayashi, Riichi Takahashi, Masatsugu Ueda, Tatsuyuki Nakashima, Naomi Nakagata.
Abstract
We attempted to cryopreserve spermatozoa from closed colonies (Jcl:SD and Jcl:Wistar), and inbred (BN/Crj, F3441 DuCrj, LEW/Crj, Long-Evans and WKY/NCrj), mutant (Zitter [WTC.ZI-zi] and Tremor [TRM]), transgenic (human A-transferase [A], and green fluorescent protein [GFP]) strains of rats. Rat epididymal spermatozoa suspended in cryopreservation solution (23% egg yolk, 8% lactose monohydrate, and 0.7% Equex Stm, pH 7.4, adjusted with 10% Tris [hydroxymethy] aminomethane) were frozen and stored at -196 degrees C. After thawing at 37 degrees C, the spermatozoa were instilled into the tip of each uterine horn of the recipients. A total of five recipient females for each strain were inseminated with cryopreserved spermatozoa, and normal live offspring of all strains (Jcl:SD: 11, Jcl:Wistar: 13, BN/Crj: 9, F344/DuCrj: 28, LEW/Crj: 4, Long-Evans: 6, WKY/NCrj: 8, TRM: 24, WTC.ZI-zi: 27, A: 30 and GFP: 20) were obtained.Entities:
Mesh：
Year: 2003 PMID： 14727812
Source DB: PubMed Journal: Comp Med ISSN： 1532-0820 Impact factor: 0.982