PURPOSE: To investigate a simplified panel of fluorescent in-situ hybridization (FISH) probes for evaluation of patients with chronic lymphocytic leukemia (CLL) and to correlate results from this technique with known prognostic factors. METHODS: We retrospectively reviewed the FISH and conventional cytogenetic results, and clinical and laboratory data of 44 patients with CLL. RESULTS: FISH was more sensitive than conventional cytogenetics in detecting genomic aberrations (75% vs. 16%, P < 0.0001). Trisomy 12 was significantly correlated with the cell surface marker of CD38 expression (P = 0.0017). CONCLUSION: This FISH panel reliably detects prognostically important genomic abnormalities in CLL and is suitable for widespread use.
PURPOSE: To investigate a simplified panel of fluorescent in-situ hybridization (FISH) probes for evaluation of patients with chronic lymphocytic leukemia (CLL) and to correlate results from this technique with known prognostic factors. METHODS: We retrospectively reviewed the FISH and conventional cytogenetic results, and clinical and laboratory data of 44 patients with CLL. RESULTS: FISH was more sensitive than conventional cytogenetics in detecting genomic aberrations (75% vs. 16%, P < 0.0001). Trisomy 12 was significantly correlated with the cell surface marker of CD38 expression (P = 0.0017). CONCLUSION: This FISH panel reliably detects prognostically important genomic abnormalities in CLL and is suitable for widespread use.
Authors: Stephanie A Smoley; Daniel L Van Dyke; Neil E Kay; Nyla A Heerema; Marie L Dell' Aquila; Paola Dal Cin; Prasad Koduru; Ayala Aviram; Laura Rassenti; John C Byrd; Kanti R Rai; Jennifer R Brown; Andrew W Greaves; Jeanette Eckel-Passow; Donna Neuberg; Thomas J Kipps; Gordon W Dewald Journal: Cancer Genet Cytogenet Date: 2010-12
Authors: Eiman A Al Zaabi; Louis A Fernandez; Irene A Sadek; D Christie Riddell; Wenda L Greer Journal: J Mol Diagn Date: 2010-01-21 Impact factor: 5.568