| Literature DB >> 14726229 |
Raphael Hirata1, Sonia M S Souza, Cláudio M Rocha-de-Souza, Arnaldo F B Andrade, Luiz H Monteiro-Leal, Luiz C D Formiga, Ana L Mattos-Guaraldi.
Abstract
Corynebacterium diphtheriae strains displayed different degrees of attachment to HEp-2 cell monolayers with two distinct adherence patterns, termed localised (LA) and diffuse (DA). The LA phenotype predominated over the DA phenotype. The non-sucrose fermenting strains expressing DA pattern adhered mostly with high index values (> or =10bact/cell). Low adhesion index (<10bact/cell) was mainly observed among sucrose fermenting strains. The fluorescein isothiocyanate (FITC)-labelled phalloidin assay (fluorescent-actin staining test) showed positive results for microorganisms of both LA and DA phenotypes. The FITC-labelled C. diphtheriae non-fimbrial surface proteins 67-72p interacted directly with HEp-2 cell membranes. Therefore, toxigenic C. diphtheriae exhibited LA and DA adherence patterns and ability to induce actin polymerisation. The experimental evidences also pointed to 67-72p as putative adhesins of C. diphtheriae to HEp-2 cells.Entities:
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Year: 2004 PMID： 14726229 DOI： 10.1016/j.micpath.2003.10.002
Source DB: PubMed Journal: Microb Pathog ISSN： 0882-4010 Impact factor: 3.738