PROBLEM: Nuclear factor kappa B (NF-kappaB) is one candidate transcriptional modulator, which might regulate many kinds of molecules that play sequential roles at implantation in the endometrium. However, temporal and spatial activation of NF-kappaB at implantation window is unknown. METHODS: Activation of NF-kappaB in the mouse uterus was determined by electrophoretic mobility shift assays. Localization of p50 and p65, components of NF-kappaB, was analyzed by immunohistochemistry. RESULTS: NF-kappaB was activated in the proestrus and estrus phases in non-pregnant uterus. In the pregnant uterus, NF-kappaB was activated after day 1.5 post-coitum, and the activation continued during implantation period. The immunoreactivities of p50 and p65 were mainly localized in endometrial epithelium, and were weaker in endometrial stroma cells. CONCLUSION: NF-kappaB activity is dynamically regulated during the sexual cycle as well as during the implantation period in the endometrium, where the biochemical interaction between mother and conceptus first occurs.
PROBLEM: Nuclear factor kappa B (NF-kappaB) is one candidate transcriptional modulator, which might regulate many kinds of molecules that play sequential roles at implantation in the endometrium. However, temporal and spatial activation of NF-kappaB at implantation window is unknown. METHODS: Activation of NF-kappaB in the mouse uterus was determined by electrophoretic mobility shift assays. Localization of p50 and p65, components of NF-kappaB, was analyzed by immunohistochemistry. RESULTS:NF-kappaB was activated in the proestrus and estrus phases in non-pregnant uterus. In the pregnant uterus, NF-kappaB was activated after day 1.5 post-coitum, and the activation continued during implantation period. The immunoreactivities of p50 and p65 were mainly localized in endometrial epithelium, and were weaker in endometrial stroma cells. CONCLUSION:NF-kappaB activity is dynamically regulated during the sexual cycle as well as during the implantation period in the endometrium, where the biochemical interaction between mother and conceptus first occurs.
Authors: Koji Yoshinaga; Mercy PrabhuDas; Christopher Davies; Kenneth White; Kathleen Caron; Thaddeus Golos; Asgerally Fazleabas; Bibhash Paria; Gil Mor; Soumen Paul; Xiaoqin Ye; Sudhansu K Dey; Thomas Spencer; Robert Michael Roberts Journal: Am J Reprod Immunol Date: 2013-11-29 Impact factor: 3.886
Authors: Jason W Ross; Morgan D Ashworth; Daniel Mathew; Patrick Reagan; Jerry W Ritchey; Kanako Hayashi; Thomas E Spencer; Matthew Lucy; Rodney D Geisert Journal: Reprod Biol Endocrinol Date: 2010-04-28 Impact factor: 5.211