Pulses of cell Ca(2+) and the dynamics of tight junction opening and closing.

A mathematical modeling of tight junction (TJ) dynamics was elaborated in a previous study to better understand the dynamics of TJ opening and closing, as well as oscillations of TJ permeability that are observed in response to changes of extracellular Ca(2+) levels. In this model, TJs were assumed to be specifically controlled by the Ca(2+) concentration levels at the extracellular Ca(2+) binding sites of zonula adhaerens. Despite the fact that the model predicts all aspects of TJ dynamics, we cannot rule out the likelihood that changes of intracellular Ca(2+) concentration (Ca(2+) (cell)), which might result from changes \ of extracellular Ca(2+) concentration (Ca(2+) (extl)), contribute to the observed results. In order to address this aspect of TJ regulation, fast Ca(2+)-switch experiments were performed in which changes of Ca(2+) (cell) were induced using the Ca(2+) ionophore A23187 or thapsigargin, a specific inhibitor of the sarco-endoplasmic reticulum Ca(2+)-ATPase. The results indicate that the ionophore or thapsigargin per se do not affect basal tissue electrical conductance ( G), showing that the sealing of TJs is not affected by a rise in Ca(2+) (cell). When TJs were kept in a dynamic state, as partially open structures or in oscillation, conditions in which the junctions are very sensitive to disturbances that affect their regulation, a rise of Ca(2+) (cell) never led to a decline of G, indicating that a rise of Ca(2+) (cell) does not trigger per se TJ closure. On the contrary, always the first response to a rise of Ca(2+) (cell) is an increase of G that, in most cases, is a transient response. Despite these observations we cannot assure that a rise of Ca(2+) (cell) is without effect on the TJs, since an increase of Ca(2+) (cell) not only causes a transient increase of G but, in addition, during oscillations a rise of Ca(2+) (cell) induced by the Ca(2+) ionophore transiently halted the oscillatory pattern of TJs. The main conclusion of this study is that TJ closure that is observed when basolateral Ca(2+) concentration (Ca(2+) (bl)) is increased after TJs were opened by Ca(2+) (bl) removal cannot be ascribed to a rise of Ca(2+) (cell) and might be a consequence of Ca(2+) binding to extracellular Ca(2+) sites.