BACKGROUND: Hephaestin is a multicopper ferroxidase required for basolateral transport of iron from enterocytes. Sex linked anaemia (sla) mice have a defect in the release of iron from intestinal enterocytes into the circulation due to an interstitial deletion in the hephaestin gene (heph). RESULTS: We have demonstrated that hephaestin is primarily localised to a supranuclear compartment in both intestinal enterocytes and in cultured cells. In normal intestinal enterocytes, hephaestin was also present on the basolateral surface. In sla mice, hephaestin was present only in the supranuclear compartment. In contrast, the iron permease Ireg1 localised to the basolateral membrane in both control and sla mice. CONCLUSION: We suggest that mislocalisation of hephaestin likely contributes to the functional defect in sla intestinal epithelium.
BACKGROUND:Hephaestin is a multicopper ferroxidase required for basolateral transport of iron from enterocytes. Sex linked anaemia (sla) mice have a defect in the release of iron from intestinal enterocytes into the circulation due to an interstitial deletion in the hephaestin gene (heph). RESULTS: We have demonstrated that hephaestin is primarily localised to a supranuclear compartment in both intestinal enterocytes and in cultured cells. In normal intestinal enterocytes, hephaestin was also present on the basolateral surface. In sla mice, hephaestin was present only in the supranuclear compartment. In contrast, the iron permease Ireg1 localised to the basolateral membrane in both control and sla mice. CONCLUSION: We suggest that mislocalisation of hephaestin likely contributes to the functional defect in sla intestinal epithelium.
Authors: A T McKie; P Marciani; A Rolfs; K Brennan; K Wehr; D Barrow; S Miret; A Bomford; T J Peters; F Farzaneh; M A Hediger; M W Hentze; R J Simpson Journal: Mol Cell Date: 2000-02 Impact factor: 17.970
Authors: C D Vulpe; Y M Kuo; T L Murphy; L Cowley; C Askwith; N Libina; J Gitschier; G J Anderson Journal: Nat Genet Date: 1999-02 Impact factor: 38.330
Authors: Huijun Chen; Zouhair K Attieh; Basharut A Syed; Yien-Ming Kuo; Valerie Stevens; Brie K Fuqua; Henriette S Andersen; Claire E Naylor; Robert W Evans; Lorraine Gambling; Ruth Danzeisen; Mhenia Bacouri-Haidar; Julnar Usta; Chris D Vulpe; Harry J McArdle Journal: J Nutr Date: 2010-08-04 Impact factor: 4.798