Yao-dong Jiang1, Shao-bin Zheng, Zhan-hui Wang. 1. Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. jyd@fimmu.com
Abstract
OBJECTIVE: To evaluate the reliability of reverse transcription (RT)-PCR in detecting the expression of the carbonic anhydrase IX (MN/CAIX) gene in renal cell carcinoma (RCC). METHODS: MN/CAIX was examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAIX was designed and two-step RT-PCR of the extracted total RNA performed. The products was examined by 2% agarose electrophoresis and identified by sequence analysis. RESULTS: Specific bands of MN/CAIX were found in RT-PCR products of 4 case of RCC tissues, whereas could not be seen in normal renal tissues. CONCLUSION: RT-PCR is reliable for detecting the expression of MN/CAIX gene in RCC.
OBJECTIVE: To evaluate the reliability of reverse transcription (RT)-PCR in detecting the expression of the carbonic anhydrase IX (MN/CAIX) gene in renal cell carcinoma (RCC). METHODS: MN/CAIX was examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAIX was designed and two-step RT-PCR of the extracted total RNA performed. The products was examined by 2% agarose electrophoresis and identified by sequence analysis. RESULTS: Specific bands of MN/CAIX were found in RT-PCR products of 4 case of RCC tissues, whereas could not be seen in normal renal tissues. CONCLUSION: RT-PCR is reliable for detecting the expression of MN/CAIX gene in RCC.