AIM: To observe the effect of beta-ionone on the proliferation of human gastric adenocarcinoma cell line SGC-7901 and the inhibition of metalloproteinase. METHODS: Using growth inhibition, Zymograms assays and reverse transcription-polymerase-chain reaction (RT-PCR), we examined cell growth rates, activities of matrix metalloproteinases-2 (MMP-2) and -9 (MMP-9), and expression of metalloproteinases-1 (TIMP-1) and -2 (TIMP-2) in SGC-7901 cells after the treatment with beta-ionone for 24 h and 48 h, respectively. RESULTS: beta-ionone had an inhibitory effect on the growth of SGC-7901 cells. Eight days after the treatment with beta-ionone at concentrations of 25, 50, 100 and 200 micromol/L, the inhibition rates were 25.9%, 28.2%, 74.4% and 90.1%, respectively. The IC50 value of beta-ionone for SGC-7901 cells was estimated to be 89 micromol/L. The effects of beta-ionone on MMP-2 and MMP-9 activities in SGC-7901 cells were not observed. However, the levels of TIMP-1 and TIMP-2 transcripts were elevated in cells treated with beta-ionone in a dose-dependent manner. CONCLUSION: beta-ionone can inhibit the proliferation of SGC-7901 cells, upregulate the expression of TIMP-1 and TIMP-2 expression, and may influence metastasis of cancer.
AIM: To observe the effect of beta-ionone on the proliferation of humangastric adenocarcinoma cell line SGC-7901 and the inhibition of metalloproteinase. METHODS: Using growth inhibition, Zymograms assays and reverse transcription-polymerase-chain reaction (RT-PCR), we examined cell growth rates, activities of matrix metalloproteinases-2 (MMP-2) and -9 (MMP-9), and expression of metalloproteinases-1 (TIMP-1) and -2 (TIMP-2) in SGC-7901 cells after the treatment with beta-ionone for 24 h and 48 h, respectively. RESULTS:beta-ionone had an inhibitory effect on the growth of SGC-7901 cells. Eight days after the treatment with beta-ionone at concentrations of 25, 50, 100 and 200 micromol/L, the inhibition rates were 25.9%, 28.2%, 74.4% and 90.1%, respectively. The IC50 value of beta-ionone for SGC-7901 cells was estimated to be 89 micromol/L. The effects of beta-ionone on MMP-2 and MMP-9 activities in SGC-7901 cells were not observed. However, the levels of TIMP-1 and TIMP-2 transcripts were elevated in cells treated with beta-ionone in a dose-dependent manner. CONCLUSION:beta-ionone can inhibit the proliferation of SGC-7901 cells, upregulate the expression of TIMP-1 and TIMP-2 expression, and may influence metastasis of cancer.
Authors: Pratima Nangia-Makker; Victor Hogan; Yuichiro Honjo; Sara Baccarini; Larry Tait; Robert Bresalier; Avraham Raz Journal: J Natl Cancer Inst Date: 2002-12-18 Impact factor: 13.506
Authors: M D Sternlicht; A Lochter; C J Sympson; B Huey; J P Rougier; J W Gray; D Pinkel; M J Bissell; Z Werb Journal: Cell Date: 1999-07-23 Impact factor: 41.582