Literature DB >> 14715765

Comparative analysis of multilocus sequence typing and pulsed-field gel electrophoresis for characterizing Listeria monocytogenes strains isolated from environmental and clinical sources.

Tamara Revazishvili1, Mamuka Kotetishvili, O Colin Stine, Arnold S Kreger, J Glenn Morris, Alexander Sulakvelidze.   

Abstract

One hundred seventy-five Listeria monocytogenes strains were characterized by serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) based on loci in actA, betL, hlyA, gyrB, pgm, and recA. One hundred twenty-two sequence types (STs) were identified by MLST based on allelic profiles of the four housekeeping genes (betL, gyrB, pgm, and recA), and 34 and 38 alleles were identified for hlyA and actA, respectively. Several actA and hlyA alleles appeared to be predominantly associated with clinical isolates. MLST differentiated most of the L. monocytogenes strains better than did PFGE, and the discriminating ability of PFGE was better than that of serotyping. Several strains with different serotypes were found, by MLST and PFGE, to have very closely related genetic backgrounds, which suggested possible "antigen switching" among them. MLST can be a useful typing tool for differentiating L. monocytogenes strains (including strains undistinguishable by PFGE typing and serotyping), and it may be of value during investigations of food-borne outbreaks of listeriosis.

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Year:  2004        PMID： 14715765      PMCID： PMC321703          DOI： 10.1128/JCM.42.1.276-285.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  36 in total

1.  Multilocus sequence typing for characterization of clinical and environmental salmonella strains.

Authors:  Mamuka Kotetishvili; O Colin Stine; Arnold Kreger; J Glenn Morris; Alexander Sulakvelidze
Journal:  J Clin Microbiol       Date:  2002-05       Impact factor: 5.948

2.  In vitro and in vivo invasiveness of different pulsed-field gel electrophoresis types of Listeria monocytogenes.

Authors:  Charlotte Nexmann Larsen; Birgit Nørrung; Helle Mølgaard Sommer; Mogens Jakobsen
Journal:  Appl Environ Microbiol       Date:  2002-11       Impact factor: 4.792

3.  Genetic homogeneity among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes.

Authors:  Z W Jaradat; G E Schutze; A K Bhunia
Journal:  Int J Food Microbiol       Date:  2002-06-05       Impact factor: 5.277

4.  Correlations between molecular subtyping and serotyping of Listeria monocytogenes.

Authors:  C A Nadon; D L Woodward; C Young; F G Rodgers; M Wiedmann
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

5.  Comparative genomics of Listeria species.

Authors:  P Glaser; L Frangeul; C Buchrieser; C Rusniok; A Amend; F Baquero; P Berche; H Bloecker; P Brandt; T Chakraborty; A Charbit; F Chetouani; E Couvé; A de Daruvar; P Dehoux; E Domann; G Domínguez-Bernal; E Duchaud; L Durant; O Dussurget; K D Entian; H Fsihi; F García-del Portillo; P Garrido; L Gautier; W Goebel; N Gómez-López; T Hain; J Hauf; D Jackson; L M Jones; U Kaerst; J Kreft; M Kuhn; F Kunst; G Kurapkat; E Madueno; A Maitournam; J M Vicente; E Ng; H Nedjari; G Nordsiek; S Novella; B de Pablos; J C Pérez-Diaz; R Purcell; B Remmel; M Rose; T Schlueter; N Simoes; A Tierrez; J A Vázquez-Boland; H Voss; J Wehland; P Cossart
Journal:  Science       Date:  2001-10-26       Impact factor: 47.728

6.  Multilocus sequence typing reveals a lack of diversity among Escherichia coli O157:H7 isolates that are distinct by pulsed-field gel electrophoresis.

Authors:  Anna C Noller; M Catherine McEllistrem; O Colin Stine; J Glenn Morris; David J Boxrud; Bruce Dixon; Lee H Harrison
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

7.  Rational design of DNA sequence-based strategies for subtyping Listeria monocytogenes.

Authors:  Steven Cai; Dirce Yorika Kabuki; Arnaldo Yoshiteru Kuaye; Theresa Gina Cargioli; Michael S Chung; Rasmus Nielsen; Martin Wiedmann
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

8.  Application of multilocus enzyme electrophoresis in studies of the epidemiology of Listeria monocytogenes in Denmark.

Authors:  B Nørrung; N Skovgaard
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

Review 9.  Listeria monocytogenes, a food-borne pathogen.

Authors:  J M Farber; P I Peterkin
Journal:  Microbiol Rev       Date:  1991-09

10.  Pulsed-field fingerprinting of listeriae: identification of genomic divisions for Listeria monocytogenes and their correlation with serovar.

Authors:  R Brosch; J Chen; J B Luchansky
Journal:  Appl Environ Microbiol       Date:  1994-07       Impact factor: 4.792
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  27 in total

1.  Rapid discrimination of Listeria monocytogenes strains by microtemperature gradient gel electrophoresis.

Authors:  Tatsuya Tominaga
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

2.  Different clonal complexes of methicillin-resistant Staphylococcus aureus are disseminated in the Euregio Meuse-Rhine region.

Authors:  Ruud H Deurenberg; Cornelis Vink; Guy J Oudhuis; Jascha E Mooij; Christel Driessen; Guy Coppens; Jos Craeghs; Els De Brauwer; Sebastian Lemmen; Hans Wagenvoort; Alexander W Friedrich; Jacques Scheres; Ellen E Stobberingh
Journal:  Antimicrob Agents Chemother       Date:  2005-10       Impact factor: 5.191

3.  The presence of the internalin gene in natural atypically hemolytic Listeria innocua strains suggests descent from L. monocytogenes.

Authors:  Dmitriy V Volokhov; Sandra Duperrier; Alexander A Neverov; Joseph George; Carmen Buchrieser; Anthony D Hitchins
Journal:  Appl Environ Microbiol       Date:  2007-01-12       Impact factor: 4.792

4.  Gene Scanning of an Internalin B Gene Fragment Using High-Resolution Melting Curve Analysis as a Tool for Rapid Typing of Listeria monocytogenes.

Authors:  Ariane T Pietzka; Anna Stöger; Steliana Huhulescu; Franz Allerberger; Werner Ruppitsch
Journal:  J Mol Diagn       Date:  2010-12-23       Impact factor: 5.568

5.  Multi-virulence-locus sequence typing identifies single nucleotide polymorphisms which differentiate epidemic clones and outbreak strains of Listeria monocytogenes.

Authors:  Yi Chen; Wei Zhang; Stephen J Knabel
Journal:  J Clin Microbiol       Date:  2007-01-10       Impact factor: 5.948

6.  Suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of Listeria monocytogenes from genomic DNA.

Authors:  Monica K Borucki; James Reynolds; Douglas R Call; Todd J Ward; Brent Page; James Kadushin
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

7.  Multilocus sequence typing for studying genetic relationships among Yersinia species.

Authors:  Mamuka Kotetishvili; Arnold Kreger; Georges Wauters; J Glenn Morris; Alexander Sulakvelidze; O Colin Stine
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

8.  Isolation and characterization of Listeria monocytogenes isolates from ready-to-eat foods in Florida.

Authors:  Yuelian Shen; Yan Liu; Yifan Zhang; Jennifer Cripe; William Conway; Jianghong Meng; Grace Hall; Arvind A Bhagwat
Journal:  Appl Environ Microbiol       Date:  2006-07       Impact factor: 4.792

9.  Relatedness of Listeria monocytogenes Isolates recovered from selected ready-to-eat foods and listeriosis patients in the United States.

Authors:  Stefanie Evans Gilbreth; Jeff E Call; F Morgan Wallace; Virginia N Scott; Yuhuan Chen; John B Luchansky
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

10.  Evolution and molecular phylogeny of Listeria monocytogenes isolated from human and animal listeriosis cases and foods.

Authors:  K K Nightingale; K Windham; M Wiedmann
Journal:  J Bacteriol       Date:  2005-08       Impact factor: 3.490
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